Reference Report

Reference
Citation	Sanghavi, P., Lu, S., Gonsalvez, G.B. (2012). A functional link between localized Oskar, dynamic microtubules, and endocytosis. Dev. Biol. 367(1): 66--77. (Export to RIS)
FlyBase ID	FBrf0218511
Publication Type	Research paper
PubMed ID	22561189
PubMed Abstract	Many cell types including developing oocytes, fibroblasts, epithelia and neurons use mRNA localization as a means to establish polarity. The Drosophila oocyte has served as a useful model in dissecting the mechanism of mRNA localization. The polarity of the oocyte is established by the specific localization of three critical mRNAs-oskar, bicoid and gurken. The localization of these mRNAs requires microtubule integrity, and the activity of microtubule motors. However, the precise organization of the oocyte microtubule cytoskeleton remains an open question. In order to examine the polarity of oocyte microtubules, we visualized the localization of canonical microtubule plus end binding proteins, EB1 and CLIP-190. Both proteins were enriched at the posterior of the oocyte, with additional foci detected within the oocyte cytoplasm and along the cortex. Surprisingly, however, we found that this asymmetric distribution of EB1 and CLIP-190 was not essential for oskar mRNA localization. However, Oskar protein was required for recruiting the plus end binding proteins to the oocyte posterior. Lastly, our results suggest that the enrichment of growing microtubules at the posterior pole functions to promote high levels of endocytosis in this region of the cell. Thus, multiple polarity-determining pathways are functionally linked in the Drosophila oocytes.
DOI	10.1016/j.ydbio.2012.04.024
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (2)
	Df(2R)PC4 Df(3R)p-XT103
Alleles (12)
	cnn^{Scer\UAS.P\T.T:Avic\GFP} Ecol\lacZ^{P\T.T:Khc-606} osk² osk^{fs(1)K10.3'UTR.Scer\UAS} osk^{Scer\UAS.P\T.cZa} par-1^k06323 par-1^{N1S.Scer\UAS.P\T.T:Avic\GFP-m6} par-1^W3 Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Scer\GAL4^{nos.UTR.T:Hsim\VP16} SmD3^P01793 stau²
Constructs (7)
	P{GAL4::VP16-nos.UTR} P{KZ.TRAP} P{matα4-GAL-VP16} P{UAS-osk.K10.3'UTR} P{UASp-GFP-Cnn1} P{UASp-osk.Z} P{UASp-par-1.N1S.GFP}
Genes (14)
	CLIP-190 cnn Eb1 Ecol\lacZ Gl Khc osk par-1 Scer\GAL4 SmD3 stau vas γTub23C γTub37C