Reference Report

Reference
Citation	Berdnik, D., Favaloro, V., Luo, L. (2012). The SUMO Protease Verloren Regulates Dendrite and Axon Targeting in Olfactory Projection Neurons. J. Neurosci. 32(24): 8331--8340. (Export to RIS)
FlyBase ID	FBrf0218602
Publication Type	Research paper
PubMed ID	22699913
PubMed Abstract	Sumoylation is a post-translational modification regulating numerous biological processes. Small ubiquitin-like modifier (SUMO) proteases are required for the maturation and deconjugation of SUMO proteins, thereby either promoting or reverting sumoylation to modify protein function. Here, we show a novel role for a predicted SUMO protease, Verloren (Velo), during projection neuron (PN) target selection in the Drosophila olfactory system. PNs target their dendrites to specific glomeruli within the antennal lobe (AL) and their axons stereotypically into higher brain centers. We uncovered mutations in velo that disrupt PN targeting specificity. PN dendrites that normally target to a particular dorsolateral glomerulus instead mistarget to incorrect glomeruli within the AL or to brain regions outside the AL. velo mutant axons also display defects in arborization. These phenotypes are rescued by postmitotic expression of Velo in PNs but not by a catalytic domain mutant of Velo. Two other SUMO proteases, DmUlp1 and CG12717, can partially compensate for the function of Velo in PN dendrite targeting. Additionally, mutations in SUMO and lesswright (which encodes a SUMO conjugating enzyme) similarly disrupt PN targeting, confirming that sumoylation is required for neuronal target selection. Finally, genetic interaction studies suggest that Velo acts in SUMO deconjugation rather than in maturation. Our study provides the first in vivo evidence for a specific role of a SUMO protease during neuronal target selection that can be dissociated from its functions in neuronal proliferation and survival.
DOI	10.1523/JNEUROSCI.6574-10.2012
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Alleles (14)
	CG12717^ex6 CG12717^{Scer\UAS.T:Ivir\HA1} lwr¹³ lwr^Scer\UAS.cAa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^GH146 smt3^ex77 smt3^SH0182 Ulp1^{Scer\UAS.T:Ivir\HA1} velo^{C1624S.Scer\UAS.T:Ivir\HA1} velo^e01260 velo^LL05207 velo^LL05209 velo^{Scer\UAS.T:Ivir\HA1}
Constructs (8)
	P{UAS-CG12717-HA} P{UAS-lwr.A} P{UAS-mCD8::GFP.L} P{UAS-Ulp1-HA} P{UAS-velo-HA.C?S} P{UAS-velo-HA} PBac{RB} PBac{SAstopDsRed}
Genes (10)
	CG11023 CG12717 CG32110 lwr Mmus\Cd8a Scer\GAL4 smt3 T:Ivir\HA1 Ulp1 velo
Insertions (6)
	P{GawB}GH146 P{XP}d05069 PBac{RB}e01706a PBac{RB}velo^e01260 PBac{SAstopDsRed}LL05207 PBac{SAstopDsRed}LL05209
Natural transposons (1)
	P-element