Reference Report

Reference
Citation	Lawlor, K.T., O'Keefe, L.V., Samaraweera, S.E., van Eyk, C.L., Richards, R.I. (2012). Ubiquitous Expression of CUG or CAG Trinucleotide Repeat RNA Causes Common Morphological Defects in a Drosophila Model of RNA-Mediated Pathology. PLoS ONE 7(6): e38516. (Export to RIS)
FlyBase ID	FBrf0218612
Publication Type	Research paper
PubMed ID	22715390
PubMed Abstract	Expanded DNA repeat sequences are known to cause over 20 diseases, including Huntington's disease, several types of spinocerebellar ataxia and myotonic dystrophy type 1 and 2. A shared genetic basis, and overlapping clinical features for some of these diseases, indicate that common pathways may contribute to pathology. Multiple mechanisms, mediated by both expanded homopolymeric proteins and expanded repeat RNA, have been identified by the use of model systems, that may account for shared pathology. The use of such animal models enables identification of distinct pathways and their 'molecular hallmarks' that can be used to determine the contribution of each pathway in human pathology. Here we characterise a tergite disruption phenotype in adult flies, caused by ubiquitous expression of either untranslated CUG or CAG expanded repeat RNA. Using the tergite phenotype as a quantitative trait we define a new genetic system in which to examine 'hairpin' repeat RNA-mediated cellular perturbation. Further experiments use this system to examine whether pathways involving Muscleblind sequestration or Dicer processing, which have been shown to mediate repeat RNA-mediated pathology in other model systems, contribute to cellular perturbation in this model.
DOI	10.1371/journal.pone.0038516
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Alleles (15)
	Avic\GFP^{Scer\UAS.T:Zzzz\CAG-100} Avic\GFP^{Scer\UAS.T:Zzzz\CAG-CAA.100} Avic\GFP^{Scer\UAS.T:Zzzz\CAG-CTG.100} Dcr-1^Q1147X Dcr-2^L811fsX mbl^E27 Scer\GAL4^Act5C.PI Scer\GAL4^da.G32 Scer\GAL4^T155 T:Zzzz\CAG¹⁰⁰ T:Zzzz\CAG^CAA.100 T:Zzzz\CAG^CTG.100 Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}
Constructs (8)
	P{Act5C-GAL4} P{GAL4-da.G32} P{UAS-CAA.94} P{UAS-CAG.99} P{UAS-rCAA.100.GFP} P{UAS-rCAG.100.GFP} P{UAS-rCUG.100.GFP} P{UAS-rCUG.100}
Genes (7)
	Avic\GFP Dcr-1 Dcr-2 mbl Scer\GAL4 T:Zzzz\CAG Zzzz\CAG
Insertions (1)
	P{GawB}T155
Natural transposons (1)
	P-element