Reference Report

Reference
Citation	Lin, C.H., Paulson, A., Abmayr, S.M., Workman, J.L. (2012). HP1a Targets the Drosophila KDM4A Demethylase to a Subset of Heterochromatic Genes to Regulate H3K36me3 Levels. PLoS ONE 7(6): e39758. (Export to RIS)
FlyBase ID	FBrf0218736
Publication Type	Research paper
PubMed ID	22761891
PubMed Abstract	The KDM4 subfamily of JmjC domain-containing demethylases mediates demethylation of histone H3K36me3/me2 and H3K9me3/me2. Several studies have shown that human and yeast KDM4 proteins bind to specific gene promoters and regulate gene expression. However, the genome-wide distribution of KDM4 proteins and the mechanism of genomic-targeting remain elusive. We have previously identified Drosophila KDM4A (dKDM4A) as a histone H3K36me3 demethylase that directly interacts with HP1a. Here, we performed H3K36me3 ChIP-chip analysis in wild type and dkdm4a mutant embryos to identify genes regulated by dKDM4A demethylase activity in vivo. A subset of heterochromatic genes that show increased H3K36me3 levels in dkdm4a mutant embryos overlap with HP1a target genes. More importantly, binding to HP1a is required for dKDM4A-mediated H3K36me3 demethylation at a subset of heterochromatic genes. Collectively, these results show that HP1a functions to target the H3K36 demethylase dKDM4A to heterochromatic genes in Drosophila.
DOI	10.1371/journal.pone.0039758
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Alleles (3)
	Kdm4A^KG04636 Kdm4A^T:Zzzz\FLAG Kdm4A^{V423A.T:Zzzz\FLAG}
Constructs (2)
	P{Flag-Kdm4A.V423A} P{Flag-Kdm4A}
Genes (11)
	CG17374 CG17514 CG40006 Cht3 His3 Kdm4A lt MFS17 Scp1 Su(var)205 T:Zzzz\FLAG
Natural transposons (1)
	P-element