Reference Report

Reference
Citation	Laflamme, C., Assaker, G., Ramel, D., Dorn, J.F., She, D., Maddox, P.S., Emery, G. (2012). Evi5 promotes collective cell migration through its Rab-GAP activity. J. Cell Biol. 198(1): 57--67. (Export to RIS)
FlyBase ID	FBrf0218839
Publication Type	Research paper
PubMed ID	22778279
PubMed Abstract	Membrane trafficking has well-defined roles during cell migration. However, its regulation is poorly characterized. In this paper, we describe the first screen for putative Rab-GTPase-activating proteins (GAPs) during collective cell migration of Drosophila melanogaster border cells (BCs), identify the uncharacterized Drosophila protein Evi5 as an essential membrane trafficking regulator, and describe the molecular mechanism by which Evi5 regulates BC migration. Evi5 requires its Rab-GAP activity to fulfill its functions during migration and acts as a GAP protein for Rab11. Both loss and gain of Evi5 function blocked BC migration by disrupting the Rab11-dependent polarization of active guidance receptors. Altogether, our findings deepen our understanding of the molecular machinery regulating endocytosis and subsequently cell signaling during migration.
DOI	10.1083/jcb.201112114
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Alleles (72)
	CG1695^GD9243 CG1695^GD16830 CG1695^KK112032 CG4041^GD11198 CG4041^KK101865 CG4552^GD11348 CG4552^KK101438 CG5337^GD11612 CG5337^KK106854 CG5745^GD11756 CG5745^KK101191 CG5916^KK109366 CG6182^GD6249 CG6182^KK100522 CG7324^HMS00722 CG7742^GD9953 CG7742^KK104814 CG8155^GD13821 CG8155^KK100131 CG8449^GD13930 CG12241^HMS00612 CG16896^GD9228 CG16896^KK100326 CG17883^GD3551 CG32506^JF03204 CG42795^GD7836 CG42795^KK112367 Evi5^GD6735 Evi5^KK102267 Evi5^{R160A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} Evi5^{R160A.Scer\UAS.T:Disc\RFP-mCherry} Evi5^{Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} Evi5^{Scer\UAS.T:Disc\RFP-mCherry} GAPcenA^GD12136 GAPcenA^KK101516 GAPcenA^Scer\UAS.cLa GAPsec^GD9897 GAPsec^KK101310 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Msp-300^GD9539 Msp-300^GD10317 Msp-300^GD10406 Msp-300^KK112156 Msp-300^KK113643 Muc14A^KK105893 plx^GD7129 plx^KK100306 Rab3-GAP^GD12118 Rab3-GAP^KK101062 Rab5^{Q88L.Scer\UAS.N.T:Avic\GFP-EGFP} Rab7^{Q67L.Scer\UAS.N.T:Avic\GFP-EGFP} Rab10^{Q68L.Scer\UAS.N.T:Avic\GFP-EGFP} Rab11^ex1 Rab11^{Q70L.Scer\UAS.N.T:Avic\GFP-YFP.Venus} Rab11^{S25N.Scer\UAS} Rab23^{Q96L.Scer\UAS.N.T:Avic\GFP-EGFP} Rab35^{Q67L.Scer\UAS.N.T:Avic\GFP-EGFP} Rip11^{Scer\UAS.C.T:Avic\GFP-EGFP} RN-tre^GD12373 RN-tre^KK102661 Scer\GAL4^c306 Scer\GAL4^slbo.2.6 sec15^{C.Scer\UAS.T:Avic\GFP} sky^GD14193 sky^KK107614 T:Avic\GFP^EGFP T:Avic\GFP^YFP.Venus T:Disc\RFP^mCherry Tbc1d15-17^GD7314 Tbc1d15-17^KK102715 Tbc1d15-17^Scer\UAS.cLa wkd^GD11617
Constructs (66)
	P{GAL4-slbo.2.6} P{GD3551} P{GD6249} P{GD6735} P{GD7129} P{GD7836} P{GD9228} P{GD9243} P{GD9539} P{GD9897} P{GD9953} P{GD10317} P{GD10406} P{GD11198} P{GD11348} P{GD11612} P{GD11617} P{GD11756} P{GD12118} P{GD12136} P{GD12373} P{GD13821} P{GD13930} P{GD14193} P{GD16830} P{KK100131} P{KK100306} P{KK100326} P{KK100522} P{KK101062} P{KK101191} P{KK101310} P{KK101438} P{KK101516} P{KK101865} P{KK102267} P{KK102661} P{KK102715} P{KK104814} P{KK105893} P{KK106854} P{KK107614} P{KK109366} P{KK112032} P{KK112156} P{KK112367} P{KK113643} P{TRiP.HMS00612} P{TRiP.HMS00722} P{TRiP.JF03204} P{UAS-EGFP-Rab5.Q88L} P{UAS-EGFP-Rab7.Q67L} P{UAS-EGFP-Rab10.Q68L} P{UAS-EGFP-Rab23.Q96L} P{UAS-EGFP-Rab35.Q67L} P{UAS-Evi5-Cherry.R160A} P{UAS-Evi5-Cherry} P{UAS-Evi5-EGFP-HA.R160A} P{UAS-Evi5-EGFP-HA} P{UAS-GapcenA.L} P{UAS-mCD8::GFP.L} P{UAS-Rab11.S25N} P{UAS-Rip11-EGFP} P{UAS-sec15.GFP} P{UAS-Tbc1d15-17.L} P{UAS-YFP-Rab11.Q70L}
Genes (39)
	CG1695 CG4041 CG4552 CG5337 CG5745 CG5916 CG6182 CG7324 CG7742 CG8155 CG8449 CG12241 CG16896 CG17883 CG32506 CG42795 Evi5 GAPcenA GAPsec Mmus\Cd8a Msp-300 Muc14A plx Rab3-GAP Rab4 Rab5 Rab7 Rab10 Rab11 Rab23 Rab35 Rip11 RN-tre Scer\GAL4 sec15 sky T:Ivir\HA1 Tbc1d15-17 wkd
Insertions (1)
	P{GawB}c306
Natural transposons (1)
	P-element