Home
Reference Report
| Reference | |||
|---|---|---|---|
| Citation | Bejarano, F., Bortolamiol-Becet, D., Dai, Q., Sun, K., Saj, A., Chou, Y.T., Raleigh, D.R., Kim, K., Ni, J.Q., Duan, H., Yang, J.S., Fulga, T.A., Van Vactor, D., Perrimon, N., Lai, E.C. (2012). A genome-wide transgenic resource for conditional expression of Drosophila microRNAs. Development 139(15): 2821--2831. (Export to RIS) | ||
| FlyBase ID | FBrf0218840 | ||
| Publication Type | Research paper | ||
| PubMed ID | 22745315 | ||
| PubMed Abstract | microRNAs (miRNAs) are endogenous short RNAs that mediate vast networks of post-transcriptional gene regulation. Although computational searches and experimental profiling provide evidence for hundreds of functional targets for individual miRNAs, such data rarely provide clear insight into the phenotypic consequences of manipulating miRNAs in vivo. We describe a genome-wide collection of 165 Drosophila miRNA transgenes and find that a majority induced specific developmental defects, including phenocopies of mutants in myriad cell-signaling and patterning genes. Such connections allowed us to validate several likely targets for miRNA-induced phenotypes. Importantly, few of these phenotypes could be predicted from computationally predicted target lists, thus highlighting the value of whole-animal readouts of miRNA activities. Finally, we provide an example of the relevance of these data to miRNA loss-of-function conditions. Whereas misexpression of several K box miRNAs inhibited Notch pathway activity, reciprocal genetic interaction tests with miRNA sponges demonstrated endogenous roles of the K box miRNA family in restricting Notch signaling. In summary, we provide extensive evidence that misexpression of individual miRNAs often induces specific mutant phenotypes that can guide their functional study. By extension, these data suggest that the deregulation of individual miRNAs in other animals may frequently yield relatively specific phenotypes during disease conditions. | ||
| DOI | 10.1242/dev.079939 | ||
| Related Publication(s) | |||
| Supplementary material | Table S2. Detailed descriptions of miRNA gain-of-function phenotypes. [FBrf0219415] |
||
| Personal communication to FlyBase | Unpublished UAS-mir lines from the Transgenic RNAi Project. Transgenic RNAi Project, 2012.9.7, Unpublished UAS-mir lines from the Transgenic RNAi Project. [FBrf0219416] |
||
Recent Updates
|
|||
| Description |
What does this section display?
This section contains items that were added to this record for each release.
It currently only tracks new links between this FlyBase report and other
FlyBase data classes (e.g. genes, references, stocks) or controlled
vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
|
||
| Update Feed |
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your
feed reader.
|
||
| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
|
Associated Information
|
|||
| Comments | |||
| Associated Files | |||
|
Other Information
|
|||
| Secondary IDs | |||
| Language of Publication | English | ||
| Additional Languages of Abstract | |||
| Also Published As | |||
|
Parent Publication
|
|||
| Publication Type | Journal | ||
| Abbreviation | Development | ||
| Title | Development | ||
| Publication Year | 1987- | ||
| ISBN/ISSN | 0950-1991 | ||
|
Data from Reference
|
|||
| Alleles (74)
|
|||
|
|||
| Constructs (13)
|
|||
|
|||
| Genes (62)
|
|||
|
|||
| Insertions (4)
|
|||
| Natural transposons (1)
|
|||