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Citation
Timofeev, K., Joly, W., Hadjieconomou, D., Salecker, I. (2012). Localized netrins act as positional cues to control layer-specific targeting of photoreceptor axons in Drosophila.  Neuron 75(1): 80--93.
FlyBase ID
FBrf0218874
Publication Type
Research paper
Abstract

A shared feature of many neural circuits is their organization into synaptic layers. However, the mechanisms that direct neurites to distinct layers remain poorly understood. We identified a central role for Netrins and their receptor Frazzled in mediating layer-specific axon targeting in the Drosophila visual system. Frazzled is expressed and cell autonomously required in R8 photoreceptors for directing their axons to the medulla-neuropil layer M3. Netrin-B is specifically localized in this layer owing to axonal release by lamina neurons L3 and capture by target neuron-associated Frazzled. Ligand expression in L3 is sufficient to rescue R8 axon-targeting defects of Netrin mutants. R8 axons target normally despite replacement of diffusible Netrin-B by membrane-tethered ligands. Finally, Netrin localization is instructive because expression in ectopic layers can retarget R8 axons. We propose that provision of localized chemoattractants by intermediate target neurons represents a highly precise strategy to direct axons to a positionally defined layer.

PubMed ID
PubMed Central ID
PMC3398394 (PMC) (EuropePMC)
Related Publication(s)
Note

Axon trapping: constructing the visual system one layer at a time.
Schwabe and Clandinin, 2012, Neuron 75(1): 6--8 [FBrf0218895]

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neuron
    Title
    Neuron
    Publication Year
    1988-
    ISBN/ISSN
    0896-6273
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    Aberrations (1)
    Alleles (30)
    Genes (14)
    Natural transposons (1)
    Insertions (11)
    Experimental Tools (5)
    Transgenic Constructs (17)
    Transcripts (13)