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Citation
Yao, Z., Macara, A.M., Lelito, K.R., Minosyan, T.Y., Shafer, O.T. (2012). Analysis of functional neuronal connectivity in the Drosophila brain.  J. Neurophysiol. 108(2): 684--696.
FlyBase ID
FBrf0218936
Publication Type
Research paper
Abstract

Drosophila melanogaster is a valuable model system for the neural basis of complex behavior, but an inability to routinely interrogate physiologic connections within central neural networks of the fly brain remains a fundamental barrier to progress in the field. To address this problem, we have introduced a simple method of measuring functional connectivity based on the independent expression of the mammalian P2X2 purinoreceptor and genetically encoded Ca(2+) and cAMP sensors within separate genetically defined subsets of neurons in the adult brain. We show that such independent expression is capable of specifically rendering defined sets of neurons excitable by pulses of bath-applied ATP in a manner compatible with high-resolution Ca(2+) and cAMP imaging in putative follower neurons. Furthermore, we establish that this approach is sufficiently sensitive for the detection of excitatory and modulatory connections deep within larval and adult brains. This technically facile approach can now be used in wild-type and mutant genetic backgrounds to address functional connectivity within neuronal networks governing a wide range of complex behaviors in the fly. Furthermore, the effectiveness of this approach in the fly brain suggests that similar methods using appropriate heterologous receptors might be adopted for other widely used model systems.

PubMed ID
PubMed Central ID
PMC3404787 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Neurophysiol.
    Title
    Journal of Neurophysiology
    Publication Year
    1938-
    ISBN/ISSN
    0022-3077
    Data From Reference