Reference Report

Reference
Citation	Chakrabarti, S., Liehl, P., Buchon, N., Lemaitre, B. (2012). Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut. Cell Host Microbe 12(1): 60--70. (Export to RIS)
FlyBase ID	FBrf0218984
Publication Type	Research paper
PubMed ID	22817988
PubMed Abstract	Typically, immune responses control the pathogen, while repair and stress pathways limit damage caused by pathogenesis. The relative contribution of damage to the outcome of pathogenesis and the mechanistic links between the immune and repair pathways are poorly understood. Here, we analyze how the entomopathogenic bacterium Pseudomonas entomophila induces irreversible damage to the Drosophila gut. We find that P. entomophila ingestion induces a global translational blockage that impairs both immune and repair programs in the fly gut. P. entomophila-induced translational inhibition is dependent on bacterial pore forming toxins and reactive oxygen species produced by the host in response to infection. Translational arrest is mediated through activation of the GCN2 kinase and inhibition of the TOR pathway as a consequence of host damage. Together, our study draws a model of pathogenesis in which bacterial inhibition of translation by excessive activation of stress responsive pathways inhibits both immune and regenerative epithelial responses.
DOI	10.1016/j.chom.2012.06.001
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell Host Microbe
Title	Cell Host & Microbe
Publication Year	2007--
ISBN/ISSN	1931-3128 1934-6069
Data from Reference
Alleles (16)
	chico¹ Dpt^T:Zzzz\His6 Duox^{dsRNA.Scer\UAS} Ecol\lacZ^Dpt.PR Ecol\lacZ^Hsp26.-351 Ecol\lacZ^puc-E69 Gcn2^KK103566 gig^NIG.6975R Gαq¹ Mekk1^Ur36 Scer\GAL4^Act.PU Scer\GAL4^da.G32 Scer\GAL4^{Myo31DF-NP0001} Scer\GAL80^ts.αTub84B SNF1A³ Tor^NIG.5092R
Constructs (9)
	P{cP-351} P{Dipt2.2-lacZ} P{Dpt-His} P{GAL4-da.G32} P{KK103566} P{NIG.5092R} P{NIG.6975R} P{tubP-GAL80^ts} P{UAS-Duox.RNAi}
Genes (55)
	aos Ark AttA CG4835 chico Cry Dcp-1 Dpt Drsl3 Duox Ecol\lacZ eIF-2α Ercc1 Gadd45 Gal Gcn2 gig GstD5 GstD6 Gαq Hsp26 Hsp70Ab InR Jon65Aiv Jra Krn Ku80 Lip3 Mdr50 Mekk1 Mtk NijA p38c p53 Peritrophin-15b Prx2540-1 puc Pvf1 Scer\GAL4 Scer\GAL80 scyl Ser8 sima SNF1A Socs36E T:Zzzz\His6 th Thor Tor TotA Try29F upd2 upd3 vn βTry
Insertions (1)
	P{GawB}Myo31DF^NP0001
Natural transposons (1)
	P-element