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McDonnell, C.M., King, D., Comeron, J.M., Li, H., Sun, W., Berenbaum, M.R., Schuler, M.A., Pittendrigh, B.R. (2012). Evolutionary toxicogenomics: diversification of the cyp12d1 and cyp12d3 genes in Drosophila species.  J. Mol. Evol. 74(5-6): 281--296.
FlyBase ID
FBrf0219004
Publication Type
Research paper
Abstract
Gene duplication and divergence are overwhelmingly considered to be the primary mechanisms by which cytochrome P450 monooxygenases (P450s) have radiated into a large and diverse gene superfamily. To address how environmental stress drives the fixation and diversification of gene duplications, we have analyzed Cyp12d1 and Cyp12d3, a pair of duplicated genes found in the sequenced Drosophila genomes of the melanogaster group. The paralog Cyp12d3, which is not found in Drosophila melanogaster, is basal to the melanogaster group, after it split from the obscura group (ca. 50 mya), and has a significant signature of positive selection in two species (D. sechellia and D. ananassae). Examination of the Cyp12d1 region in D. melanogaster wildtype and isoline populations revealed variation both in copy number and sequence, including splice-site variations, which certainly alter gene function. Further investigations of several strains have identified three cases in which differences in the Cyp12d1 gene region are associated with the differences in transcript abundance and transcriptional responses to the environmental stresses that have not been seen for other detoxificative loci. Together, these data highlight the value of using both macro- and microevolutionary approaches in studying the duplication and divergence events associated with detoxification genes and lay important groundwork for future studies in the field of evolutionary toxicogenomics, which uses the principles of phylogenetic analysis to predict possible enzymatic functions.
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Mol. Evol.
    Title
    Journal of Molecular Evolution
    Publication Year
    1971-
    ISBN/ISSN
    0022-2844
    Data From Reference
    Genes (3)