Reference Report

Reference
Citation	Palm, W., Sampaio, J.L., Brankatschk, M., Carvalho, M., Mahmoud, A., Shevchenko, A., Eaton, S. (2012). Lipoproteins in Drosophila melanogaster-Assembly, Function, and Influence on Tissue Lipid Composition. PLoS Genet. 8(7): e1002828. (Export to RIS)
FlyBase ID	FBrf0219024
Publication Type	Research paper
PubMed ID	22844248
PubMed Abstract	Interorgan lipid transport occurs via lipoproteins, and altered lipoprotein levels correlate with metabolic disease. However, precisely how lipoproteins affect tissue lipid composition has not been comprehensively analyzed. Here, we identify the major lipoproteins of Drosophila melanogaster and use genetics and mass spectrometry to study their assembly, interorgan trafficking, and influence on tissue lipids. The apoB-family lipoprotein Lipophorin (Lpp) is the major hemolymph lipid carrier. It is produced as a phospholipid-rich particle by the fat body, and its secretion requires Microsomal Triglyceride Transfer Protein (MTP). Lpp acquires sterols and most diacylglycerol (DAG) at the gut via Lipid Transfer Particle (LTP), another fat body-derived apoB-family lipoprotein. The gut, like the fat body, is a lipogenic organ, incorporating both de novo-synthesized and dietary fatty acids into DAG for export. We identify distinct requirements for LTP and Lpp-dependent lipid mobilization in contributing to the neutral and polar lipid composition of the brain and wing imaginal disc. These studies define major routes of interorgan lipid transport in Drosophila and uncover surprising tissue-specific differences in lipoprotein lipid utilization.
DOI	10.1371/journal.pgen.1002828
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS Genet.
Title	PLoS Genetics
Publication Year	2005-
ISBN/ISSN	1553-7404 1553-7390
Data from Reference
Alleles (28)
	AkhR¹ AkhR^GD586 Apoltp^Dex1C Apoltp^{dsRNA.Scer\FRT.Scer\UAS.I} Apoltp^{dsRNA.Scer\FRT.Scer\UAS.II} Apoltp^{dsRNA.Scer\UAS.I} Apoltp^{dsRNA.Scer\UAS.II} Apoltp^Δex1A bmm¹ bmm^GD5139 CG3523^GD14739 cv-d^GD1820 Dcr-2^Scer\UAS.cDa Mtp^{dsRNA.Scer\FRT.Scer\UAS} Mtp^{dsRNA.Scer\UAS} Mtp^Scer\UAS.cPa Mtp^Δex1 Rfabg^{dsRNA.ApoLI.Scer\UAS} Rfabg^{dsRNA.ApoLII.Scer\UAS} Rfabg^{dsRNA.Scer\FRT.ApoLI.Scer\UAS} Rfabg^{dsRNA.Scer\FRT.ApoLII.Scer\UAS} Rfabg^{Scer\UAS.T:Ivir\HA1,T:Hsap\MYC} Scer\GAL4^Adh.PF Scer\GAL4^{Myo31DF-NP0001} Scer\GAL4^Rfabg.PB Scer\GAL4^tub.PU Scer\GAL80^ts.αTub84B shi^{K44A.Scer\UAS}
Constructs (23)
	P{EP} P{GAL4-Adh.Dmul} P{GD586} P{GD1820} P{GD5139} P{GD14739} P{Lpp-GAL4.B} P{tub-GAL4.U} P{tubP-GAL80^ts} P{UAS(FRT.Hcri\RFP)Apoltp.dsRNA.1} P{UAS(FRT.Hcri\RFP)Apoltp.dsRNA.II} P{UAS(FRT.Hcri\RFP)Mtp.dsRNA} P{UAS(FRT.T:Hcri\RFP)ApoLI.RNAi} P{UAS(FRT.T:Hcri\RFP)ApoLII.RNAi} P{UAS(-FRT)ApoLI.RNAi} P{UAS(-FRT)ApoLII.RNAi} P{UAS(-FRT)Apoltp.dsRNA.1} P{UAS(-FRT)Apoltp.dsRNA.II} P{UAS(-FRT)Mtp.dsRNA} P{UAS-Dcr-2.D} P{UAS-HA-ApoL-Myc} P{UAS-Mtp.P} P{UAS-shi.K44A}
Genes (11)
	AkhR Apoltp bmm CG3523 cv-d Dcr-2 Mtp Rfabg Scer\GAL4 Scer\GAL80 shi
Insertions (3)
	P{EP}GE10702 P{GawB}Myo31DF^NP0001 P{GT1}BG01038
Natural transposons (1)
	P-element