Reference Report

Reference
Citation	Nagel, J., Delandre, C., Zhang, Y., Förstner, F., Moore, A.W., Tavosanis, G. (2012). Fascin controls neuronal class-specific dendrite arbor morphology. Development 139(16): 2999--3009. (Export to RIS)
FlyBase ID	FBrf0219030
Publication Type	Research paper
PubMed ID	22764047
PubMed Abstract	The branched morphology of dendrites represents a functional hallmark of distinct neuronal types. Nonetheless, how diverse neuronal class-specific dendrite branches are generated is not understood. We investigated specific classes of sensory neurons of Drosophila larvae to address the fundamental mechanisms underlying the formation of distinct branch types. We addressed the function of fascin, a conserved actin-bundling protein involved in filopodium formation, in class III and class IV sensory neurons. We found that the terminal branchlets of different classes of neurons have distinctive dynamics and are formed on the basis of molecularly separable mechanisms; in particular, class III neurons require fascin for terminal branching whereas class IV neurons do not. In class III neurons, fascin controls the formation and dynamics of terminal branchlets. Previous studies have shown that transcription factor combinations define dendrite patterns; we find that fascin represents a downstream component of such programs, as it is a major effector of the transcription factor Cut in defining class III-specific dendrite morphology. Furthermore, fascin defines the morphological distinction between class III and class IV neurons. In fact, loss of fascin function leads to a partial conversion of class III neurons to class IV characteristics, while the reverse effect is obtained by fascin overexpression in class IV neurons. We propose that dedicated molecular mechanisms underlie the formation and dynamics of distinct dendrite branch types to elicit the accurate establishment of neuronal circuits.
DOI	10.1242/dev.077800
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (17)
	Act5C^{Scer\UAS.T:Avic\GFP} Avic\GFP^1.E.ppk ct^Scer\UAS.cPa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Mmus\Cd8a^{Scer\UAS.T:Disc\RFP-mCherry} Rac1^Scer\UAS.cLa Scer\GAL4^80G2 Scer\GAL4^109(2)80 Scer\GAL4²²¹ Scer\GAL4⁴⁷⁷ Scer\GAL4^smid-C161 sn³ sn^36a sn^{S52A.Scer\UAS.T:Zzzz\KO-mKO} sn^{S52D.Scer\UAS.T:Zzzz\KO-mKO} sn^{Scer\UAS.T:Zzzz\KO-mKO} T:Zzzz\KO^mKO
Constructs (10)
	M{UAS-mKO-sn.S52A} M{UAS-mKO-sn.S52D} M{UAS-mKO-sn} P{GAL4} P{ppk-EGFP.1} P{UAS-Act5C.T:GFP} P{UAS-ct.P} P{UAS-mCD8::GFP.L} P{UAS-mCD8.ChRFP} P{UAS-Rac1.L}
Genes (7)
	Act5C Avic\GFP ct Mmus\Cd8a Rac1 Scer\GAL4 sn
Insertions (5)
	P{GAL4}80G2 P{GawB}109(2)80 P{GawB}221 P{GawB}477 P{GawB}smid^C161
Natural transposons (1)
	Dmau\mariner