Reference Report

Reference
Citation	Lewis, P.W., Sahoo, D., Geng, C., Bell, M., Lipsick, J.S., Botchan, M.R. (2012). Drosophila Lin-52 Acts in Opposition to Repressive Components of the Myb-MuvB/dREAM Complex. Mol. Cell. Biol. 32(16): 3218--3227. (Export to RIS)
FlyBase ID	FBrf0219063
Publication Type	Research paper
PubMed ID	22688510
PubMed Abstract	The Drosophila melanogaster Myb-MuvB/dREAM complex (MMB/dREAM) participates in both the activation and repression of developmentally regulated genes and origins of DNA replication. Mutants in MMB subunits exhibit diverse phenotypes, including lethality, eye defects, reduced fecundity, and sterility. Here, we used P-element excision to generate mutations in lin-52, which encodes the smallest subunit of the MMB/dREAM complex. lin-52 is required for viability, as null mutants die prior to pupariation. The generation of somatic and germ line mutant clones indicates that lin-52 is required for adult eye development and for early embryogenesis via maternal effects. Interestingly, the maternal-effect embryonic lethality, larval lethality, and adult eye defects could be suppressed by mutations in other subunits of the MMB/dREAM complex. These results suggest that a partial MMB/dREAM complex is responsible for the lethality and eye defects of lin-52 mutants. Furthermore, these findings support a model in which the Lin-52 and Myb proteins counteract the repressive activities of the other members of the MMB/dREAM complex at specific genomic loci in a developmentally controlled manner.
DOI	10.1128/MCB.00432-12
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Alleles (9)
	lin-52⁹³ lin-52^+t1.6 lin-52^T:Avic\GFP lin-52^{Ubi-p63E.T:Zzzz\FLAG} mip40^EY16520 mip120⁶⁷ mip130^1-36 Myb^MH107 W^GMR.PG
Constructs (4)
	P{GMR-hid} P{lin-52^+t1.6} P{lin-52-GFP} P{Ubi-p63E-lin-52.FLAG}
Genes (10)
	Caf1 E2f2 lin-52 mip40 mip120 mip130 Myb T:Avic\GFP T:Zzzz\FLAG W
Insertions (2)
	P{EP}EP404 P{EPgy2}mip40^EY16520
Natural transposons (1)
	P-element