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Formaz-Preston, A., Ryu, J.R., Svendsen, P.C., Brook, W.J. (2012). The Tbx20 homolog Midline represses wingless in conjunction with Groucho during the maintenance of segment polarity.  Dev. Biol. 369(2): 319--329.
FlyBase ID
FBrf0219199
Publication Type
Research paper
Abstract

The regulation of the segment polarity gene wingless is essential for the correct patterning of the Drosophila ectoderm. We have previously shown that the asymmetric activation of wingless downstream of Hedghog-signaling depends on the T-box transcription factors, midline and H15. Hedgehog activates wingless anterior to the Hedgehog domain. midline/H15 are responsible in part for repressing wingless in cells posterior to the Hedgehog expressing cells. Here, we show that Midline binds the Groucho co-repressor directly via the engrailed homology-1 domain and requires an intact engrailed-homology-1 domain to repress wingless. In contrast, the regulation of Serrate, a second target of midline repression, is not dependent on the engrailed-homology-1 domain. Furthermore, we identify a midline responsive region of the wingless cis-regulatory region and show that Midline binds to sequences within this region. Mutating these sequences in transgenic reporter constructs results in ectopic reporter expression in the midline-expression domain, consistent with wingless being a direct target of Midline repression.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Biol.
    Title
    Developmental Biology
    Publication Year
    1959-
    ISBN/ISSN
    0012-1606
    Data From Reference
    Aberrations (1)
    Alleles (12)
    Genes (9)
    Physical Interactions (4)
    Natural transposons (1)
    Insertions (2)
    Experimental Tools (3)
    Transgenic Constructs (8)