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Takaesu, N.T., Stinchfield, M.J., Shimizu, K., Arase, M., Quijano, J.C., Watabe, T., Miyazono, K., Newfeld, S.J. (2012). Drosophila CORL is required for Smad2-mediated activation of Ecdysone Receptor expression in the mushroom body.  Development 139(18): 3392--3401.
FlyBase ID
FBrf0219224
Publication Type
Research paper
Abstract

CORL proteins (FUSSEL/SKOR proteins in humans) are related to Sno/Ski oncogenes but their developmental roles are unknown. We have cloned Drosophila CORL and show that its expression is restricted to distinct subsets of cells in the central nervous system. We generated a deletion of CORL and noted that homozygous individuals rarely survive to adulthood. Df(4)dCORL adult escapers display mushroom body (MB) defects and Df(4)dCORL larvae are lacking Ecdysone Receptor (EcR-B1) expression in MB neurons. This is phenocopied in CORL-RNAi and Smad2-RNAi clones in wild-type larvae. Furthermore, constitutively active Baboon (type I receptor upstream of Smad2) cannot stimulate EcR-B1 MB expression in Df(4)dCORL larvae, which demonstrates a formal requirement for CORL in Smad2 signaling. Studies of mouse Corl1 (Skor1) revealed that it binds specifically to Smad3. Overall, the data suggest that CORL facilitates Smad2 activity upstream of EcR-B1 in the MB. The conservation of neural expression and strong sequence homology of all CORL proteins suggests that this is a new family of Smad co-factors.

PubMed ID
PubMed Central ID
PMC4074270 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Development
    Title
    Development
    Publication Year
    1987-
    ISBN/ISSN
    0950-1991
    Data From Reference
    Aberrations (2)
    Alleles (15)
    Genes (15)
    Natural transposons (1)
    Insertions (5)
    Experimental Tools (2)
    Transgenic Constructs (10)
    Transcripts (1)