Open Close
Tsou, W.L., Sheedlo, M.J., Morrow, M.E., Blount, J.R., McGregor, K.M., Das, C., Todi, S.V. (2012). Systematic Analysis of the Physiological Importance of Deubiquitinating Enzymes.  PLoS ONE 7(8): e43112.
FlyBase ID
Publication Type
Research paper

Deubiquitinating enzymes (DUBs) are proteases that control the post-translational modification of proteins by ubiquitin and in turn regulate diverse cellular pathways. Despite a growing understanding of DUB biology at the structural and molecular level, little is known about the physiological importance of most DUBs. Here, we systematically identify DUBs encoded by the genome of Drosophila melanogaster and examine their physiological importance in vivo. Through domain analyses we uncovered 41 Drosophila DUBs, most of which have human orthologues. Systematic knockdown of the vast majority of DUBs throughout the fly or in specific cell types had dramatic consequences for Drosophila development, adult motility or longevity. Specific DUB subclasses proved to be particularly necessary during development, while others were important in adults. Several DUBs were indispensable in neurons or glial cells during developmental stages; knockdown of others perturbed the homeostasis of ubiquitinated proteins in adult flies, or had adverse effects on wing positioning as a result of neuronal requirements. We demonstrate the physiological significance of the DUB family of enzymes in intact animals, find that there is little functional redundancy among members of this family of proteases, and provide insight for future investigations to understand DUB biology at the molecular, cellular and organismal levels.

PubMed ID
PubMed Central ID
PMC3427330 (PMC) (EuropePMC)
Associated Information
Associated Files
Other Information
Secondary IDs
    Language of Publication
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    PLoS ONE
    PLoS ONE
    Publication Year
    Data From Reference
    Alleles (38)
    Gene Groups (6)
    Genes (44)
    Natural transposons (1)
    Insertions (1)
    Transgenic Constructs (37)
    Transcripts (1)