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Citation
Mandilaras, K., Missirlis, F. (2012). Genes for iron metabolism influence circadian rhythms in Drosophila melanogaster.  Metallomics 4(9): 928--936.
FlyBase ID
FBrf0219269
Publication Type
Research paper
Abstract

Haem has been previously implicated in the function of the circadian clock, but whether iron homeostasis is integrated with circadian rhythms is unknown. Here we describe an RNA interference (RNAi) screen using clock neurons of Drosophila melanogaster. RNAi is targeted to iron metabolism genes, including those involved in haem biosynthesis and degradation. The results indicate that Ferritin 2 Light Chain Homologue (Fer2LCH) is required for the circadian activity of flies kept in constant darkness. Oscillations of the core components in the molecular clock, PER and TIM, were also disrupted following Fer2LCH silencing. Other genes with a putative function in circadian biology include Transferrin-3, CG1358 (which has homology to the FLVCR haem export protein) and five genes implicated in iron-sulfur cluster biosynthesis: the Drosophila homologues of IscS (CG12264), IscU (CG9836), IscA1 (CG8198), Iba57 (CG8043) and Nubp2 (CG4858). Therefore, Drosophila genes involved in iron metabolism are required for a functional biological clock.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Metallomics
    Title
    Metallomics : integrated biometal science
    ISBN/ISSN
    1756-5901 1756-591X
    Data From Reference
    Alleles (72)
    Gene Groups (3)
    Genes (55)
    Insertions (11)
    Transgenic Constructs (68)