Reference Report

Reference
Citation	Berger, C., Harzer, H., Burkard, T.R., Steinmann, J., van der Horst, S., Laurenson, A.S., Novatchkova, M., Reichert, H., Knoblich, J.A. (2012). FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. Cell Rep. 2(2): 407--418. (Export to RIS)
FlyBase ID	FBrf0219320
Publication Type	Research paper
PubMed ID	22884370
PubMed Abstract	Drosophila neuroblasts (NBs) have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type-specific gene expression data. Here, we describe a method for isolating large numbers of pure NBs and differentiating neurons that retain both cell-cycle and lineage characteristics. We determine transcriptional profiles by mRNA sequencing and identify 28 predicted NB-specific transcription factors that can be arranged in a network containing hubs for Notch signaling, growth control, and chromatin regulation. Overexpression and RNA interference for these factors identify Klumpfuss as a regulator of self-renewal. We show that loss of Klumpfuss function causes premature differentiation and that overexpression results in the formation of transplantable brain tumors. Our data represent a valuable resource for investigating Drosophila developmental neurobiology, and the described method can be applied to other invertebrate stem cell lineages as well.
DOI	10.1016/j.celrep.2012.07.008
Related Publication(s)
Supplementary material	Extended Experimental Procedures [FBrf0219846]
Personal communication to FlyBase	Neuroblast and neuron RNA-Seq tracks. Berger, 2012.10.16, Neuroblast and neuron RNA-Seq tracks. [FBrf0219845]
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Secondary IDs
Language of Publication	English
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Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Cell Rep.
Title	Cell reports
Publication Year
ISBN/ISSN	2211-1247
Data from Reference
Alleles (40)
	Avic\GFP^{Stinger.Scer\UAS.T:nls-tra} BacA\p35^Scer\UAS.cUa bigmax^Scer\UAS.cBa CG4553^Scer\UAS.cBa CG4570^Scer\UAS.cBa CG6701^Scer\UAS.cBa CG6769^Scer\UAS.cBa CG10462^Scer\UAS.cBa CG10565^Scer\UAS.cBa CG13897^Scer\UAS.cBa CG15715^Scer\UAS.cBa CG31875^Scer\UAS.cBa Coop^Scer\UAS.cBa crc^Scer\UAS.cBa Dcr-2^Scer\UAS.cDa dpn^Scer\UAS.cBa E(spl)mγ-HLH^Scer\UAS.cBa grh^Scer\UAS.cBb ken^Scer\UAS.cBa king-tubby^Scer\UAS.cBa klu^212lR51C klu^G410 klu^{miRNA.Scer\UAS} klu^Scer\UAS.cBa l(2)k10201^Scer\UAS.cBa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} mod^Scer\UAS.cBa mTTF^Scer\UAS.cBa Rel^Scer\UAS.cBa Scer\GAL4^ase.neuro Scer\GAL4^GMR9D11 Scer\GAL4^insc-Mz1407 Scer\GAL4^pnt-14-94 Scer\GAL80^ts.αTub84B Ssb-c31a^Scer\UAS.cBa Ssrp^Scer\UAS.cBa su(Hw)^Scer\UAS.cBa TFAM^Scer\UAS.cBa wek^Scer\UAS.cBa wor^Scer\UAS.cBa
Constructs (36)
	P{ase-GAL4} P{GMR9D11-GAL4} P{tubP-GAL80^ts} P{UAS-bigmax.B} P{UAS-CG4553.B} P{UAS-CG4570.B} P{UAS-CG6701.B} P{UAS-CG6769.B} P{UAS-CG10462.B} P{UAS-CG10565.B} P{UAS-CG13897.B} P{UAS-CG15715.B} P{UAS-CG31875.B} P{UAS-Coop.B} P{UAS-crc.B} P{UAS-Dcr-2.D} P{UAS-dpn.B} P{UAS-E(spl)mγ-HLH.B} P{UAS-grh} P{UAS-ken.B} P{UAS-king-tubby.B} P{UAS-klu.B} P{UAS-klu.miRNA} P{UAS-l(2)k10201.B} P{UAS-mCD8::GFP.L} P{UAS-mod.B} P{UAS-mTTF.B} P{UAS-p35.U} P{UAS-Rel.B} P{UAS-Ssb-c31a.B} P{UAS-Ssrp.B} P{UAS-Stinger} P{UAS-su(Hw).B} P{UAS-TFAM.B} P{UAS-wek.B} P{UAS-wor.B}
Genes (45)
	aPKC ase Avic\GFP BacA\p35 bigmax brat CG4553 CG4570 CG6701 CG6769 CG10462 CG10565 CG13897 CG15715 CG31875 Coop crc Dcr-2 dpn E(spl)mγ-HLH elav grh His3 Hrb27C ken king-tubby klu l(2)k10201 lola mira Mmus\Cd8a mod mTTF numb pros Rel repo Scer\GAL4 Scer\GAL80 Ssb-c31a Ssrp su(Hw) TFAM wek wor
Insertions (2)
	P{GAL4}pnt^14-94 P{GawB}insc^Mz1407
Libraries/Collections (2)
	Knoblich_mRNA_L3_CNS_neuroblast Knoblich_mRNA_L3_CNS_neuron
Natural transposons (1)
	P-element