Open Close
Reference
Citation
Berger, C., Harzer, H., Burkard, T.R., Steinmann, J., van der Horst, S., Laurenson, A.S., Novatchkova, M., Reichert, H., Knoblich, J.A. (2012). FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal.  Cell Rep. 2(2): 407--418.
FlyBase ID
FBrf0219320
Publication Type
Research paper
Abstract

Drosophila neuroblasts (NBs) have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type-specific gene expression data. Here, we describe a method for isolating large numbers of pure NBs and differentiating neurons that retain both cell-cycle and lineage characteristics. We determine transcriptional profiles by mRNA sequencing and identify 28 predicted NB-specific transcription factors that can be arranged in a network containing hubs for Notch signaling, growth control, and chromatin regulation. Overexpression and RNA interference for these factors identify Klumpfuss as a regulator of self-renewal. We show that loss of Klumpfuss function causes premature differentiation and that overexpression results in the formation of transplantable brain tumors. Our data represent a valuable resource for investigating Drosophila developmental neurobiology, and the described method can be applied to other invertebrate stem cell lineages as well.

Graphical Abstract
Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC3828055 (PMC) (EuropePMC)
Related Publication(s)
Supplementary material

Extended Experimental Procedures [FBrf0219846]

Personal communication to FlyBase

Neuroblast and neuron RNA-Seq tracks.
Berger, 2012.10.16, Neuroblast and neuron RNA-Seq tracks. [FBrf0219845]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Rep.
    Title
    Cell reports
    ISBN/ISSN
    2211-1247
    Data From Reference
    Alleles (40)
    Genes (45)
    Datasets (7)
    Natural transposons (1)
    Insertions (2)
    Experimental Tools (1)
    Transgenic Constructs (36)
    Transcripts (1)