Reference Report

Reference
Citation	Szabad, J., Bellen, H.J., Venken, K.J.T. (2012). An assay to detect in vivo Y chromosome loss in Drosophila wing disc cells. G3 (Bethesda) 2(9): 1095--1102. (Export to RIS)
FlyBase ID	FBrf0219495
Publication Type	Research paper
PubMed ID	22973547
PubMed Abstract	Loss of the Y chromosome in Drosophila has no impact on cell viability and therefore allows us to assay the impact of environmental agents and genetic alterations on chromosomal loss. To detect in vivo chromosome loss in cells of the developing Drosophila wing primordia, we first engineered a Y chromosome with an attP docking site. By making use of the ΦC31 integrase system, we site-specifically integrated a genomic transgene encompassing the multiple wing hair (mwh) locus into this attP site, leading to a mwh(+)Y chromosome. This chromosome fully rescues the mwh mutant phenotype, an excellent recessive wing cell marker mutation. Loss of this mwh(+)Y chromosome in wing primordial cells then leads to manifestation of the mwh mutant phenotype in mwh-homozygous cells. The forming mwh clones permit us to quantify the effect of agents and genetic alterations by assaying frequency and size of the mwh mosaic spots. To illustrate the use of the mwh(+)Y loss system, the effects of four known mutagens (X-rays, colchicine, ethyl methanesulfonate, and formaldehyde) and two genetic conditions (loss- and gain-of-function lodestar mutant alleles) are documented. The procedure is simple, sensitive, and inexpensive.
DOI	10.1534/g3.112.002899
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Language of Publication	English
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Publication Type	Journal
Abbreviation	G3 (Bethesda)
Title	G3 : genes - genomes - genetics
Publication Year
ISBN/ISSN	2160-1836
Data from Reference
Aberrations (2)
	Df(3R)ED5218 Dp(2;Y)G
Alleles (9)
	lds^{Hor-D.Scer\UAS.T:Avic\GFP-CFP} lds^Hor-D lds^hor-rvP2 mwh¹ mwh^+t38 Scer\GAL4^nub-AC-62 Su(var)2-1³ Su(var)3-9^ptn T:Avic\GFP^CFP
Balancers (2)
	Dp(2;Y)G-P{CaryP}attPY Dp(2;Y)G-P{mwh.+t38}attPY
Constructs (5)
	P{CaryP} P{mwh.+t38} P{UAS-lds-CFP.Hor-D} PBac{mwh.+t38} PBac{y⁺-attP-3B}
Genes (5)
	lds mwh Scer\GAL4 Su(var)2-1 Su(var)3-9
Insertions (7)
	P{CaryP}attP1 P{CaryP}attPY P{GawB}nubbin-AC-62 P{hs-hid}Y P{mwh.+t38}attPY PBac{mwh.+t38}VK00016 PBac{y⁺-attP-3B}VK00016
Natural transposons (2)
	P-element Tni\piggyBac