Reference Report

Reference
Citation	Nie, J., Mahato, S., Mustill, W., Tipping, C., Bhattacharya, S.S., Zelhof, A.C. (2012). Cross species analysis of Prominin reveals a conserved cellular role in invertebrate and vertebrate photoreceptor cells. Dev. Biol. 371(2): 312--320. (Export to RIS)
FlyBase ID	FBrf0219508
Publication Type	Research paper
PubMed ID	22960282
PubMed Abstract	The two fundamental types of photoreceptor cells have evolved unique structures to expand the apical membrane to accommodate the phototransduction machinery, exemplified by the cilia-based outer segment of the vertebrate photoreceptor cell and the microvilli-based rhabdomere of the invertebrate photoreceptor. The morphogenesis of these compartments is integral for photoreceptor cell integrity and function. However, little is known about the elementary cellular and molecular mechanisms required to generate these compartments. Here we investigate whether a conserved cellular mechanism exists to create the phototransduction compartments by examining the functional role of a photoreceptor protein common to both rhabdomeric and ciliated photoreceptor cells, Prominin. First and foremost we demonstrate that the physiological role of Prominin is conserved between rhabdomeric and ciliated photoreceptor cells. Human Prominin1 is not only capable of rescuing the corresponding rhabdomeric Drosophila prominin mutation but also demonstrates a conserved genetic interaction with a second photoreceptor protein Eyes Shut. Furthermore, we demonstrate the Prominin homologs in vertebrate and invertebrate photoreceptors require the same structural features and post-translational modifications for function. Moreover, expression of mutant human Prominin1, associated with autosomal dominant retinal degeneration, in rhabdomeric photoreceptor cells disrupts morphogenesis in ways paralleling retinal degeneration seen in ciliated photoreceptors. Taken together, our results suggest the existence of an ancestral Prominin-directed cellular mechanism to create and model the apical membranes of the two fundamental types of photoreceptor cells into their respective phototransduction compartments.
DOI	10.1016/j.ydbio.2012.08.024
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (13)
	eys¹ Hsap\EYS^Scer\UAS.cNa Hsap\PROM1^{mut1-7.Scer\UAS} Hsap\PROM1^{R373C.Scer\UAS} Hsap\PROM1^Scer\UAS.cNa Hsap\PROM1^{ΔC.Scer\UAS.T:Avic\GFP} prom¹ prom^{mut1-4.Scer\UAS} prom^Scer\UAS.cNa prom^{ΔC.Scer\UAS.T:Avic\GFP} Scer\GAL4^GMR.PF Scer\GAL4^hs.PU Scer\GAL4^Pph13
Constructs (10)
	P{GAL4-ninaE.GMR} P{hs-GAL4.U} P{UAS-hEYS.N} P{UAS-hProm1.N} P{UAS-hProm1.R373C} P{UAS-hProm1-GFP.ΔC} P{UAS-prom.mut1-4} P{UAS-prom.mut1-7} P{UAS-prom.N} P{UAS-prom-GFP.ΔC}
Genes (7)
	eys Hsap\EYS Hsap\PROM1 Pph13 prom Scer\GAL4 T:Avic\GFP
Natural transposons (1)
	P-element
Transcripts (1)
	Scer\GAL4^Pph13RA