Reference Report

Reference
Citation	Olivieri, D., Senti, K.A., Subramanian, S., Sachidanandam, R., Brennecke, J. (2012). The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila. Mol. Cell 47(6): 954--969. (Export to RIS)
FlyBase ID	FBrf0219572
Publication Type	Research paper
PubMed ID	22902557
PubMed Abstract	In animal gonads, PIWI proteins and their bound 23-30 nt piRNAs guard genome integrity by the sequence specific silencing of transposons. Two branches of piRNA biogenesis, namely primary processing and ping-pong amplification, have been proposed. Despite an overall conceptual understanding of piRNA biogenesis, identity and/or function of the involved players are largely unknown. Here, we demonstrate an essential role for the female sterility gene shutdown in piRNA biology. Shutdown, an evolutionarily conserved cochaperone collaborates with Hsp90 during piRNA biogenesis, potentially at the loading step of RNAs into PIWI proteins. We demonstrate that Shutdown is essential for both primary and secondary piRNA populations in Drosophila. An extension of our study to previously described piRNA pathway members revealed three distinct groups of biogenesis factors. Together with data on how PIWI proteins are wired into primary and secondary processing, we propose a unified model for piRNA biogenesis.
DOI	10.1016/j.molcel.2012.07.021
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Mol. Cell
Title	Molecular Cell
Publication Year	1997-
ISBN/ISSN	1097-2765 1097-4164
Data from Reference
Aberrations (1)
	Df(2R)BSC665
Alleles (26)
	AGO3^{dsRNA.shRNA.Scer\UAS.P\T} armi^{dsRNA.shRNA.Scer\UAS.P\T} armi^GD7319 aub^{dsRNA.shRNA.Scer\UAS.P\T} aub^{T:Avic\GFP-EGFP} BoYb^{dsRNA.shRNA.Scer\UAS.P\T} Hsp83^{T:Avic\GFP-EGFP} krimp^{dsRNA.shRNA.Scer\UAS.P\T} piwi^{dsRNA.shRNA.Scer\UAS.P\T} Scer\GAL4^nos.PG Scer\GAL4^{nos.UTR.T:Hsim\VP16} Scer\GAL4^{otu.T:Hsim\VP16} Scer\GAL4^tj-NP1624 shu¹ shu² shu^{7.4.T:Avic\GFP-EGFP} shu^GL00379 shu^{K304A.7.4.T:Avic\GFP-EGFP} shu^KK102092 shu^{Q308E.7.4.T:Avic\GFP-EGFP} SoYb^{dsRNA.shRNA.Scer\UAS.P\T} spn-E^{dsRNA.shRNA.Scer\UAS.P\T} T:Avic\GFP^EGFP vas^{dsRNA.shRNA.Scer\UAS.P\T} vret^{dsRNA.shRNA.Scer\UAS.P\T} zuc^GL00111
Constructs (13)
	P{aub-EGFP} P{GAL4::VP16-nos.UTR} P{GAL4-nos.NGT} P{GD7319} P{Hsp83-EGFP} P{KK102092} P{otu-GAL4::VP16.R} P{shu-EGFP.7.4.K304A} P{shu-EGFP.7.4.Q308E} P{shu-EGFP.7.4} P{TRiP.GL00111} P{UASp-AGO3.shRNA} P{UASp-vas.shRNA}
Genes (17)
	AGO3 armi aub BoYb flam fs(1)Yb Hsp83 krimp piwi Scer\GAL4 shu SoYb spn-E tj vas vret zuc
Insertions (1)
	P{GawB}NP1624
Natural transposons (38)
	3S18 1360 1731 accord Bari1 blood BS3 Burdock copia Cr1a diver diver2 Dm88 Doc F-element flea G4 G5 G5A G-element gypsy7 gypsy8 HeT-A I-element invader1 invader3 looper1 mdg3 micropia P-element pogo Porto1 R2-element rooA Rt1a Rt1c TART-element ZAM