|Citation||Cook, K. (2012.9.12). Notes on the Exelixis deletion collection. (Export to RIS)|
|Publication Type||Personal communication to FlyBase|
|Text of Personal Communication||
Here is a little history concerning the collection of deletion strains generated at Exelixis, Inc. and described in Parks et al. (FBrf0175003).
There were several deletions isolated at Exelixis, Inc. that were lost before they could be placed into public distribution by the Bloomington Stock Center. These include:
Several deletions generated at Exelixis were not described in the paper. They were communicated to FlyBase later. They include:
Four deletions were placed into distribution by the Bloomington Stock Center, but were subsequently discarded because they were identical to other deletions (i.e. generated from the same progenitor FRT insertions):
Df(1)Exel9052 (identical to Df(1)Exel9049)
Df(1)Exel9054 (identical to Df(1)Exel9051)
Df(2L)Exel9063 (identical to Df(2L)Exel9033)
Df(3R)Exel6282 (identical to Df(3R)Exel6180)
Two deletions were lost at Bloomington because the stocks broke down - presumably by recombination between the deletion and balancer chromosomes:
Several deletion stocks were obviously no good when they arrived in Bloomington, so they were discarded before being placed in public distribution. These include:
Several deletion stocks were placed into public distribution but later tests showed the expected deletion was not present. These stocks were discarded:
It is quite likely that many of the stocks in these last two lists never carried deletions. In both the DrosDel and Bloomington deletion projects, false positive chromosomes were recovered from deletion screens that showed the expected visible phenotypes and even tested positive by PCR tests, yet did not carry the expected deletions. The nature of these false positives is not well understood, but they probably involve unanticipated chromosomal rearrangements. There had not been enough time to verify the deletions genetically at Exelixis before the stocks were placed into the public domain, so verification tests were undertaken subsequently at Bloomington to eliminate bad stocks. Under the circumstances, this was the best way to make the deletion collection available to the fly community.
As of September 2012, there are still several Exelixis deletion stocks in the Bloomington collection that have not been verified by complementation tests or tests for deleted sequences. In most cases, there are no convenient mutations available for complementation tests. As they are tested, we will discard those that fail verification tests. These stocks are distributed with the caveat that the expected deletion may not be present:
What does this section display?
What does this section not display?
This section does not currently display links that were removed or gene model changes.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Language of Publication||English|
|Additional Languages of Abstract|
|Also Published As|
|Data from Reference|