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Cook, K. (2012.9.12). Notes on the Exelixis deletion collection. 
FlyBase ID
FBrf0219608
Publication Type
Personal communication to FlyBase
Abstract
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Text of Personal Communication
Here is a little history concerning the collection of deletion strains generated at Exelixis, Inc. and described in Parks et al. (FBrf0175003).  
There were several deletions isolated at Exelixis, Inc. that were lost before they could be placed into public distribution by the Bloomington Stock Center. These include:
Df(1)Exel6232
Df(1)Exel6243
Df(1)Exel6289
Df(2L)Exel6040
Df(2L)Exel7030
Df(2L)Exel7058
Df(2L)Exel7062
Df(2L)Exel7076
Df(2L)Exel8006
Df(2L)Exel8017
Df(2L)Exel9037
Df(2R)Exel6074
Df(2R)Exel6080
Df(2R)Exel6278
Df(2R)Exel7085
Df(3R)Exel6207
Df(3R)Exel7359
Several deletions generated at Exelixis were not described in the paper.  They were communicated to FlyBase later.  They include:
Df(1)Exel9052
Df(1)Exel9053
Df(1)Exel9054
Df(1)Exel9067
Df(1)Exel9068
Df(2L)Exel6022
Df(2L)Exel9063
Df(2L)Exel9064
Df(2R)Exel6285
Df(2R)Exel8056
Df(3L)Exel9058
Df(3L)Exel9065
Df(3L)Exel9066
Df(3R)Exel6282
Df(3R)Exel8159
Four deletions were placed into distribution by the Bloomington Stock Center, but were subsequently discarded because they were identical to other deletions (i.e. generated from the same progenitor FRT insertions):
Df(1)Exel9052    (identical to Df(1)Exel9049)
Df(1)Exel9054    (identical to Df(1)Exel9051)
Df(2L)Exel9063    (identical to Df(2L)Exel9033)
Df(3R)Exel6282    (identical to Df(3R)Exel6180)
Two deletions were lost at Bloomington because the stocks broke down - presumably by recombination between the deletion and balancer chromosomes:
Df(2R)Exel7184
Df(2R)Exel7185
Several deletion stocks were obviously no good when they arrived in Bloomington, so they were discarded before being placed in public distribution.  These include:
Df(1)Exel6222
Df(1)Exel6229
Df(1)Exel6246
Df(1)Exel6247
Df(1)Exel6249
Df(1)Exel6250
Df(2R)Exel6075
Df(2R)Exel6281
Df(3R)Exel6287
Several deletion stocks were placed into public distribution but later tests showed the expected deletion was not present.  These stocks were discarded:
Df(1)Exel6224
Df(1)Exel9053
Df(1)Exel9067
Df(1)Exel9068 
Df(1)Exel7465
Df(2L)Exel6026
Df(2L)Exel6032
Df(2L)Exel6037
Df(2L)Exel7002
Df(2L)Exel7009
Df(2L)Exel7017
Df(2L)Exel7061
Df(2L)Exel7065
Df(2L)Exel7075
Df(2L)Exel7082
Df(2L)Exel8001
Df(2L)Exel8004
Df(2L)Exel8010
Df(2L)Exel8011
Df(2L)Exel8021
Df(2L)Exel8024
Df(2L)Exel8029
Df(2L)Exel8032
Df(2L)Exel9023
Df(2L)Exel9027
Df(2R)Exel6073
Df(2R)Exel6081
Df(2R)Exel7112
Df(2R)Exel7129
Df(2R)Exel7133
Df(2R)Exel7139
Df(2R)Exel7147
Df(2R)Exel7152
Df(2R)Exel7163
Df(2R)Exel7167
Df(2R)Exel7174
Df(2R)Exel7176
Df(2R)Exel7179
Df(2R)Exel7180
Df(2R)Exel7182
Df(2R)Exel8048
Df(2R)Exel8049
Df(2R)Exel9021
Df(2R)Exel9022
Df(2R)Exel9024
Df(3L)Exel6100
Df(3L)Exel6111
Df(3L)Exel6113
Df(3L)Exel6124
Df(3L)Exel6136
Df(3L)Exel6139
Df(3L)Exel6261
Df(3L)Exel9005
Df(3L)Exel9007
Df(3R)Exel6175
Df(3R)Exel6177
Df(3R)Exel6219
Df(3R)Exel6220
Df(3R)Exel6258
Df(3R)Exel6260
Df(3R)Exel6266
Df(3R)Exel6268
Df(3R)Exel6271
Df(3R)Exel6286
Df(3R)Exel7323
Df(3R)Exel8156
Df(3R)Exel8160
Df(3R)Exel9055
It is quite likely that many of the stocks in these last two lists never carried deletions.  In both the DrosDel and Bloomington deletion projects, false positive chromosomes were recovered from deletion screens that showed the expected visible phenotypes and even tested positive by PCR tests, yet did not carry the expected deletions.  The nature of these false positives is not well understood, but they probably involve unanticipated chromosomal rearrangements.  There had not been enough time to verify the deletions genetically at Exelixis before the stocks were placed into the public domain, so verification tests were undertaken subsequently at Bloomington to eliminate bad stocks. Under the circumstances, this was the best way to make the deletion collection available to the fly community.
As of September 2012, there are still several Exelixis deletion stocks in the Bloomington collection that have not been verified by complementation tests or tests for deleted sequences.  In most cases, there are no convenient mutations available for complementation tests.  As they are tested, we will discard those that fail verification tests.  These stocks are distributed with the caveat that the expected deletion may not be present:
Df(1)Exel6291
Df(2L)Exel6018
Df(2L)Exel7029
Df(2L)Exel7071
Df(2L)Exel8028
Df(2L)Exel8036
Df(2L)Exel9040
Df(2R)Exel6283
Df(2R)Exel7096
Df(2R)Exel7137
Df(2R)Exel7164
Df(2R)Exel7169
Df(2R)Exel7178
Df(3L)Exel6090
Df(3L)Exel6262
Df(3L)Exel9001
Df(3L)Exel9057
Df(3R)Exel6156
Df(3R)Exel6173
Df(3R)Exel7320
Df(3R)Exel9019
Df(2L)Exel9064
DOI
Related Publication(s)
Personal communication to FlyBase
The expected deletion is not present in Df(2L)Exel9064.
Cook and Popodi, 2017.1.5, The expected deletion is not present in Df(2L)Exel9064. [FBrf0234454]
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