Here is a little history concerning the collection of deletion strains generated at Exelixis, Inc. and described in Parks et al. (FBrf0175003). There were several deletions isolated at Exelixis, Inc. that were lost before they could be placed into public distribution by the Bloomington Stock Center. These include: Df(1)Exel6232 Df(1)Exel6243 Df(1)Exel6289 Df(2L)Exel6040 Df(2L)Exel7030 Df(2L)Exel7058 Df(2L)Exel7062 Df(2L)Exel7076 Df(2L)Exel8006 Df(2L)Exel8017 Df(2L)Exel9037 Df(2R)Exel6074 Df(2R)Exel6080 Df(2R)Exel6278 Df(2R)Exel7085 Df(3R)Exel6207 Df(3R)Exel7359 Several deletions generated at Exelixis were not described in the paper. They were communicated to FlyBase later. They include: Df(1)Exel9052 Df(1)Exel9053 Df(1)Exel9054 Df(1)Exel9067 Df(1)Exel9068 Df(2L)Exel6022 Df(2L)Exel9063 Df(2L)Exel9064 Df(2R)Exel6285 Df(2R)Exel8056 Df(3L)Exel9058 Df(3L)Exel9065 Df(3L)Exel9066 Df(3R)Exel6282 Df(3R)Exel8159 Four deletions were placed into distribution by the Bloomington Stock Center, but were subsequently discarded because they were identical to other deletions (i.e. generated from the same progenitor FRT insertions): Df(1)Exel9052 (identical to Df(1)Exel9049) Df(1)Exel9054 (identical to Df(1)Exel9051) Df(2L)Exel9063 (identical to Df(2L)Exel9033) Df(3R)Exel6282 (identical to Df(3R)Exel6180) Two deletions were lost at Bloomington because the stocks broke down - presumably by recombination between the deletion and balancer chromosomes: Df(2R)Exel7184 Df(2R)Exel7185 Several deletion stocks were obviously no good when they arrived in Bloomington, so they were discarded before being placed in public distribution. These include: Df(1)Exel6222 Df(1)Exel6229 Df(1)Exel6246 Df(1)Exel6247 Df(1)Exel6249 Df(1)Exel6250 Df(2R)Exel6075 Df(2R)Exel6281 Df(3R)Exel6287 Several deletion stocks were placed into public distribution but later tests showed the expected deletion was not present. These stocks were discarded: Df(1)Exel6224 Df(1)Exel9053 Df(1)Exel9067 Df(1)Exel9068 Df(1)Exel7465 Df(2L)Exel6026 Df(2L)Exel6032 Df(2L)Exel6037 Df(2L)Exel7002 Df(2L)Exel7009 Df(2L)Exel7017 Df(2L)Exel7061 Df(2L)Exel7065 Df(2L)Exel7075 Df(2L)Exel7082 Df(2L)Exel8001 Df(2L)Exel8004 Df(2L)Exel8010 Df(2L)Exel8011 Df(2L)Exel8021 Df(2L)Exel8024 Df(2L)Exel8029 Df(2L)Exel8032 Df(2L)Exel9023 Df(2L)Exel9027 Df(2R)Exel6073 Df(2R)Exel6081 Df(2R)Exel7112 Df(2R)Exel7129 Df(2R)Exel7133 Df(2R)Exel7139 Df(2R)Exel7147 Df(2R)Exel7152 Df(2R)Exel7163 Df(2R)Exel7167 Df(2R)Exel7174 Df(2R)Exel7176 Df(2R)Exel7179 Df(2R)Exel7180 Df(2R)Exel7182 Df(2R)Exel8048 Df(2R)Exel8049 Df(2R)Exel9021 Df(2R)Exel9022 Df(2R)Exel9024 Df(3L)Exel6100 Df(3L)Exel6111 Df(3L)Exel6113 Df(3L)Exel6124 Df(3L)Exel6136 Df(3L)Exel6139 Df(3L)Exel6261 Df(3L)Exel9005 Df(3L)Exel9007 Df(3R)Exel6175 Df(3R)Exel6177 Df(3R)Exel6219 Df(3R)Exel6220 Df(3R)Exel6258 Df(3R)Exel6260 Df(3R)Exel6266 Df(3R)Exel6268 Df(3R)Exel6271 Df(3R)Exel6286 Df(3R)Exel7323 Df(3R)Exel8156 Df(3R)Exel8160 Df(3R)Exel9055 It is quite likely that many of the stocks in these last two lists never carried deletions. In both the DrosDel and Bloomington deletion projects, false positive chromosomes were recovered from deletion screens that showed the expected visible phenotypes and even tested positive by PCR tests, yet did not carry the expected deletions. The nature of these false positives is not well understood, but they probably involve unanticipated chromosomal rearrangements. There had not been enough time to verify the deletions genetically at Exelixis before the stocks were placed into the public domain, so verification tests were undertaken subsequently at Bloomington to eliminate bad stocks. Under the circumstances, this was the best way to make the deletion collection available to the fly community. As of September 2012, there are still several Exelixis deletion stocks in the Bloomington collection that have not been verified by complementation tests or tests for deleted sequences. In most cases, there are no convenient mutations available for complementation tests. As they are tested, we will discard those that fail verification tests. These stocks are distributed with the caveat that the expected deletion may not be present: Df(1)Exel6291 Df(2L)Exel6018 Df(2L)Exel7029 Df(2L)Exel7071 Df(2L)Exel8028 Df(2L)Exel8036 Df(2L)Exel9040 Df(2R)Exel6283 Df(2R)Exel7096 Df(2R)Exel7137 Df(2R)Exel7164 Df(2R)Exel7169 Df(2R)Exel7178 Df(3L)Exel6090 Df(3L)Exel6262 Df(3L)Exel9001 Df(3L)Exel9057 Df(3R)Exel6156 Df(3R)Exel6173 Df(3R)Exel7320 Df(3R)Exel9019 Df(2L)Exel9064
