Open Close
Reference
Citation
Comeron, J.M., Ratnappan, R., Bailin, S. (2012). The Many Landscapes of Recombination in Drosophila melanogaster.  PLoS Genet. 8(10): e1002905.
FlyBase ID
FBrf0219727
Publication Type
Research paper
Abstract
Recombination is a fundamental biological process with profound evolutionary implications. Theory predicts that recombination increases the effectiveness of selection in natural populations. Yet, direct tests of this prediction have been restricted to qualitative trends due to the lack of detailed characterization of recombination rate variation across genomes and within species. The use of imprecise recombination rates can also skew population genetic analyses designed to assess the presence and mode of selection across genomes. Here we report the first integrated high-resolution description of genomic and population variation in recombination, which also distinguishes between the two outcomes of meiotic recombination: crossing over (CO) and gene conversion (GC). We characterized the products of 5,860 female meioses in Drosophila melanogaster by genotyping a total of 139 million informative SNPs and mapped 106,964 recombination events at a resolution down to 2 kilobases. This approach allowed us to generate whole-genome CO and GC maps as well as a detailed description of variation in recombination among individuals of this species. We describe many levels of variation in recombination rates. At a large-scale (100 kb), CO rates exhibit extreme and highly punctuated variation along chromosomes, with hot and coldspots. We also show extensive intra-specific variation in CO landscapes that is associated with hotspots at low frequency in our sample. GC rates are more uniformly distributed across the genome than CO rates and detectable in regions with reduced or absent CO. At a local scale, recombination events are associated with numerous sequence motifs and tend to occur within transcript regions, thus suggesting that chromatin accessibility favors double-strand breaks. All these non-independent layers of variation in recombination across genomes and among individuals need to be taken into account in order to obtain relevant estimates of recombination rates, and should be included in a new generation of population genetic models of the interaction between selection and linkage.
PubMed ID
PubMed Central ID
PMC3469467 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
Ultra-high resolution recombination map for D. melanogaster.
Comeron, 2012.10.15, Ultra-high resolution recombination map for D. melanogaster. [FBrf0233439]
FlyBase analysis
Calculation of genetic map positions for D. melanogaster genes from an ultra-high-resolution whole-genome recombination map.
dos Santos, 2016.9.14, Calculation of genetic map positions for D. melanogaster genes from an ultra-high-resolution whole-genome recombination map. [FBrf0233438]
Note
Classical Genetics Meets Next-Generation Sequencing: Uncovering a Genome-Wide Recombination Map in Drosophila melanogaster.
Singh, 2012, PLoS Genet. 8(10): e1003024 [FBrf0219755]
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
  • FBrf0219772
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
PLoS Genet.
Title
PLoS Genetics
Publication Year
2005-
ISBN/ISSN
1553-7404 1553-7390
Data From Reference
Genes (17428)
List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.