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Citation
Ji, J.Y., Miles, W.O., Korenjak, M., Zheng, Y., Dyson, N.J. (2012). In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila.  G3 (Bethesda) 2(12): 1651--1660.
FlyBase ID
FBrf0220353
Publication Type
Research paper
Abstract
The E2F transcription factors are important regulators of the cell cycle whose function is commonly misregulated in cancer. To identify novel regulators of E2F1 activity in vivo, we used Drosophila to conduct genetic screens. For this, we generated transgenic lines that allow the tissue-specific depletion of dE2F1 by RNAi. Expression of these transgenes using Gal4 drivers in the eyes and wings generated reliable and modifiable phenotypes. We then conducted genetic screens testing the capacity of Exelixis deficiencies to modify these E2F1-RNAi phenotypes. From these screens, we identified mutant alleles of Suppressor of zeste 2 [Su(z)2] and multiple Polycomb group genes as strong suppressors of the E2F1-RNA interference phenotypes. In validation of our genetic data, we find that depleting Su(z)2 in cultured Drosophila cells restores the cell-proliferation defects caused by reduction of dE2F1 by elevating the level of dE2f1. Furthermore, analyses of methylation status of histone H3 lysine 27 (H3K27me) from the published modENCODE data sets suggest that the genomic regions harboring dE2f1 gene and certain dE2f1 target genes display H3K27me during development and in several Drosophila cell lines. These in vivo observations suggest that the Polycomb group may regulate cell proliferation by repressing the transcription of dE2f1 and certain dE2F1 target genes. This mechanism may play an important role in coordinating cellular differentiation and proliferation during Drosophila development.
PubMed ID
PubMed Central ID
PMC3516486 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
P{ptc-GAL4.G}2 and P{UAS-E2f1.dsRNA.J}8 insertions.
Guarner and Dyson, 2019.4.16, P{ptc-GAL4.G}2 and P{UAS-E2f1.dsRNA.J}8 insertions. [FBrf0242257]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    G3 (Bethesda)
    Title
    G3 : genes - genomes - genetics
    ISBN/ISSN
    2160-1836
    Data From Reference
    Aberrations (42)
    Alleles (66)
    Genes (37)
    Cell Lines (4)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (1)
    Transgenic Constructs (10)