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Citation
Pinder, B.D., Smibert, C.A. (2013). microRNA-independent recruitment of Argonaute 1 to nanos mRNA through the Smaug RNA-binding protein.  EMBO Rep. 14(1): 80--86.
FlyBase ID
FBrf0220359
Publication Type
Research paper
Abstract

Argonaute (Ago) proteins are typically recruited to target messenger RNAs via an associated small RNA such as a microRNA (miRNA). Here, we describe a new mechanism of Ago recruitment through the Drosophila Smaug RNA-binding protein. We show that Smaug interacts with the Ago1 protein, and that Ago1 interacts with and is required for the translational repression of the Smaug target, nanos mRNA. The Ago1/nanos mRNA interaction does not require a miRNA, but it does require Smaug. Taken together, our data suggest a model whereby Smaug directly recruits Ago1 to nanos mRNA in a miRNA-independent manner, thereby repressing translation.

PubMed ID
PubMed Central ID
PMC3537145 (PMC) (EuropePMC)
Related Publication(s)
Note

Smaug: An unexpected journey into the mechanisms of post-transcriptional regulation.
Pinder and Smibert, 2013, Fly 7(3): 142--145 [FBrf0223125]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    EMBO Rep.
    Title
    EMBO Reports
    Publication Year
    2000-
    ISBN/ISSN
    1469-221X 1469-3178
    Data From Reference
    Alleles (5)
    Genes (14)
    Physical Interactions (9)
    Natural transposons (1)
    Experimental Tools (3)
    Transgenic Constructs (2)