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Citation
Rohrbaugh, M., Clore, A., Davis, J., Johnson, S., Jones, B., Jones, K., Kim, J., Kithuka, B., Lunsford, K., Mitchell, J., Mott, B., Ramos, E., Tchedou, M.R., Acosta, G., Araujo, M., Cushing, S., Duffy, G., Graves, F., Griffin, K., Gurudatta, B.V., Jackson, D., Jaimes, D., Jamison, K., Jones, K., Kelley, D., Kilgore, M., Laramore, D., Le, T., Mazhar, B., Mazhar, M.M., McCrary, B., Miller, T., Moreland, C., Mullins, A., Munye, E., Okoorie, S., Pittman, E., Roberts, N., Rose, D., Rowland, A., Shagarabi, A., Smith, J., Stallworth, T., Stroud, N., Sung, E., Sung, K., Takenaka, N., Torre, E., Veira, J., Vu, K., Wagstaff, W., Wood, A.M., Wu, K., Yang, J., Corces, V.G. (2013). Identification and Characterization of Proteins Involved in Nuclear Organization Using Drosophila GFP Protein Trap Lines.  PLoS ONE 8(1): e53091.
FlyBase ID
FBrf0220647
Publication Type
Research paper
Abstract
Strains from a collection of Drosophila GFP protein trap lines express GFP in the normal tissues where the endogenous protein is present. This collection can be used to screen for proteins distributed in the nucleus in a non-uniform pattern.We analyzed four lines that show peripheral or punctate nuclear staining. One of these lines affects an uncharacterized gene named CG11138. The CG11138 protein shows a punctate distribution in the nuclear periphery similar to that of Drosophila insulator proteins but does not co-localize with known insulators. Interestingly, mutations in Lamin proteins result in alterations in CG11138 localization, suggesting that this protein may be a novel component of the nuclear lamina. A second line affects the Decondensation factor 31 (Df31) gene, which encodes a protein with a unique nuclear distribution that appears to segment the nucleus into four different compartments. The X-chromosome of males is confined to one of these compartments. We also find that Drosophila Nucleoplasmin (dNlp) is present in regions of active transcription. Heat shock leads to loss of dNlp from previously transcribed regions of polytene chromosome without redistribution to the heat shock genes. Analysis of Stonewall (Stwl), a protein previously found to be necessary for the maintenance of germline stem cells, shows that Stwl is present in a punctate pattern in the nucleus that partially overlaps with that of known insulator proteins. Finally we show that Stwl, dNlp, and Df31 form part of a highly interactive network. The properties of other components of this network may help understand the role of these proteins in nuclear biology.These results establish screening of GFP protein trap alleles as a strategy to identify factors with novel cellular functions. Information gained from the analysis of CG11138 Stwl, dNlp, and Df31 sets the stage for future studies of these proteins.
PubMed ID
PubMed Central ID
PMC3547006 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS ONE
    Title
    PLoS ONE
    Publication Year
    2006-
    ISBN/ISSN
    1932-6203
    Data From Reference