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Wang, C.I., Alekseyenko, A.A., Leroy, G., Elia, A.E., Gorchakov, A.A., Britton, L.M., Elledge, S.J., Kharchenko, P.V., Garcia, B.A., Kuroda, M.I. (2013). Chromatin proteins captured by ChIP-mass spectrometry are linked to dosage compensation in Drosophila.  Nat. Struct. Mol. Biol. 20(2): 202--209.
FlyBase ID
FBrf0220693
Publication Type
Research paper
Abstract

X-chromosome dosage compensation by the MSL (male-specific lethal) complex is required in Drosophila melanogaster to increase gene expression from the single male X to equal that of both female X chromosomes. Instead of focusing solely on protein complexes released from DNA, here we used chromatin-interacting protein MS (ChIP-MS) to identify MSL interactions on cross-linked chromatin. We identified MSL-enriched histone modifications, including histone H4 Lys16 acetylation and histone H3 Lys36 methylation, and CG4747, a putative Lys36-trimethylated histone H3 (H3K36me3)-binding protein. CG4747 is associated with the bodies of active genes, coincident with H3K36me3, and is mislocalized in the Set2 mutant lacking H3K36me3. CG4747 loss of function in vivo results in partial mislocalization of the MSL complex to autosomes, and RNA interference experiments confirm that CG4747 and Set2 function together to facilitate targeting of the MSL complex to active genes, validating the ChIP-MS approach.

PubMed ID
PubMed Central ID
PMC3674866 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Struct. Mol. Biol.
    Title
    Nature Structural and Molecular Biology
    Publication Year
    2004-
    ISBN/ISSN
    1545-9993 1545-9985
    Data From Reference
    Aberrations (1)
    Alleles (9)
    Genes (41)
    Natural transposons (2)
    Insertions (1)
    Experimental Tools (4)
    Transgenic Constructs (5)