Open Close
Reference
Citation
Buster, D.W., Daniel, S.G., Nguyen, H.Q., Windler, S.L., Skwarek, L.C., Peterson, M., Roberts, M., Meserve, J.H., Hartl, T., Klebba, J.E., Bilder, D., Bosco, G., Rogers, G.C. (2013). SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2.  J. Cell Biol. 201(1): 49--63.
FlyBase ID
FBrf0221230
Publication Type
Research paper
Abstract
Condensin complexes play vital roles in chromosome condensation during mitosis and meiosis. Condensin II uniquely localizes to chromatin throughout the cell cycle and, in addition to its mitotic duties, modulates chromosome organization and gene expression during interphase. Mitotic condensin activity is regulated by phosphorylation, but mechanisms that regulate condensin II during interphase are unclear. Here, we report that condensin II is inactivated when its subunit Cap-H2 is targeted for degradation by the SCF(Slimb) ubiquitin ligase complex and that disruption of this process dramatically changed interphase chromatin organization. Inhibition of SCF(Slimb) function reorganized interphase chromosomes into dense, compact domains and disrupted homologue pairing in both cultured Drosophila cells and in vivo, but these effects were rescued by condensin II inactivation. Furthermore, Cap-H2 stabilization distorted nuclear envelopes and dispersed Cid/CENP-A on interphase chromosomes. Therefore, SCF(Slimb)-mediated down-regulation of condensin II is required to maintain proper organization and morphology of the interphase nucleus.
PubMed ID
PubMed Central ID
PMC3613687 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
Comments on cell lines used, Rogers.
Rogers, 2016.4.22, Comments on cell lines used, Rogers. [FBrf0232275]
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Alleles (8)
    Genes (17)
    Physical Interactions (4)
    Cell Lines (3)
    Insertions (1)
    Transgenic Constructs (3)