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Citation
Lagha, M., Bothma, J.P., Esposito, E., Ng, S., Stefanik, L., Tsui, C., Johnston, J., Chen, K., Gilmour, D.S., Zeitlinger, J., Levine, M.S. (2013). Paused Pol II Coordinates Tissue Morphogenesis in the Drosophila Embryo.  Cell 153(5): 976--987.
FlyBase ID
FBrf0221621
Publication Type
Research paper
Abstract

Paused RNA polymerase (Pol II) is a pervasive feature of Drosophila embryos and mammalian stem cells, but its role in development is uncertain. Here, we demonstrate that a spectrum of paused Pol II determines the "time to synchrony"-the time required to achieve coordinated gene expression across the cells of a tissue. To determine whether synchronous patterns of gene activation are significant in development, we manipulated the timing of snail expression, which controls the coordinated invagination of ∼1,000 mesoderm cells during gastrulation. Replacement of the strongly paused snail promoter with moderately paused or nonpaused promoters causes stochastic activation of snail expression and increased variability of mesoderm invagination. Computational modeling of the dorsal-ventral patterning network recapitulates these variable and bistable gastrulation profiles and emphasizes the importance of timing of gene activation in development. We conclude that paused Pol II and transcriptional synchrony are essential for coordinating cell behavior during morphogenesis.

Graphical Abstract
Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC4257494 (PMC) (EuropePMC)
Related Publication(s)
Note

Taking a pause to reflect on morphogenesis.
Saunders and Ashe, 2013, Cell 153(5): 941--943 [FBrf0222233]

Gene expression: Time flies thanks to Pol II pausing.
Burgess, 2013, Nat. Rev. Genet. 14(7): 441 [FBrf0223029]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Genes (12)