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Chang, L., Kreko, T., Davison, H., Cusmano, T., Wu, Y., Rothenfluh, A., Eaton, B.A. (2013). Normal dynactin complex function during synapse growth in Drosophila requires membrane binding by Arfaptin.  Mol. Biol. Cell 24(11): 1749--1764.
FlyBase ID
FBrf0221747
Publication Type
Research paper
Abstract

Mutations in DCTN1, a component of the dynactin complex, are linked to neurodegenerative diseases characterized by a broad collection of neuropathologies. Because of the pleiotropic nature of dynactin complex function within the neuron, defining the causes of neuropathology in DCTN1 mutants has been difficult. We combined a genetic screen with cellular assays of dynactin complex function to identify genes that are critical for dynactin complex function in the nervous system. This approach identified the Drosophila homologue of Arfaptin, a multifunctional protein that has been implicated in membrane trafficking. We find that Arfaptin and the Drosophila DCTN1 homologue, Glued, function in the same pathway during synapse growth but not during axonal transport or synapse stabilization. Arfaptin physically associates with Glued and other dynactin complex components in the nervous system of both flies and mice and colocalizes with Glued at the Golgi in motor neurons. Mechanistically, membrane binding by Arfaptin mediates membrane association of the dynactin complex in motor neurons and is required for normal synapse growth. Arfaptin represents a novel dynactin complex-binding protein that specifies dynactin complex function during synapse growth.

PubMed ID
PubMed Central ID
PMC3667727 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Biol. Cell
    Title
    Molecular Biology of the Cell
    Publication Year
    1992-
    ISBN/ISSN
    1059-1524
    Data From Reference
    Aberrations (1)
    Alleles (43)
    Genes (40)
    Human Disease Models (2)
    Physical Interactions (3)
    Cell Lines (1)
    Natural transposons (1)
    Insertions (13)
    Experimental Tools (2)
    Transgenic Constructs (4)