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Citation
Mayer, L.R., Diegelmann, S., Abassi, Y., Eichinger, F., Pflugfelder, G.O. (2013). Enhancer trap infidelity in Drosophila optomotor-blind.  Fly 7(2): 118--128.
FlyBase ID
FBrf0221891
Publication Type
Research paper
Abstract

Reporter gene activity in enhancer trap lines is often implicitly assumed to mirror quite faithfully the endogenous expression of the "trapped" gene, even though there are numerous examples of enhancer trap infidelity. optomotor-blind (omb) is a 160 kb gene in which 16 independent P-element enhancer trap insertions of three different types have been mapped in a range of more than 60 kb. We have determined the expression pattern of these elements in wing, eye-antennal and leg imaginal discs as well as in the pupal tergites. We noted that one pGawB insertion (omb (P4) ) selectively failed to report parts of the omb pattern even though the missing pattern elements were apparent in all other 15 lines. We ruled out that omb (P4) was defective in the Gal4 promoter region or had inactivated genomic enhancers in the integration process. We propose that the Gal4 reporter gene in pGawB may be sensitive to orientation or promoter proximity effects.

PubMed ID
PubMed Central ID
PMC3732331 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Fly
    Title
    Fly
    Publication Year
    2007-
    ISBN/ISSN
    1933-6934 1933-6942
    Data From Reference