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Yin, Z., Sadok, A., Sailem, H., McCarthy, A., Xia, X., Li, F., Garcia, M.A., Evans, L., Barr, A.R., Perrimon, N., Marshall, C.J., Wong, S.T., Bakal, C. (2013). A screen for morphological complexity identifies regulators of switch-like transitions between discrete cell shapes.  Nat. Cell Biol. 15(7): 860--871.
FlyBase ID
FBrf0222048
Publication Type
Research paper
Abstract

The way in which cells adopt different morphologies is not fully understood. Cell shape could be a continuous variable or restricted to a set of discrete forms. We developed quantitative methods to describe cell shape and show that Drosophila haemocytes in culture are a heterogeneous mixture of five discrete morphologies. In an RNAi screen of genes affecting the morphological complexity of heterogeneous cell populations, we found that most genes regulate the transition between discrete shapes rather than generating new morphologies. In particular, we identified a subset of genes, including the tumour suppressor PTEN, that decrease the heterogeneity of the population, leading to populations enriched in rounded or elongated forms. We show that these genes have a highly conserved function as regulators of cell shape in both mouse and human metastatic melanoma cells.

PubMed ID
PubMed Central ID
PMC3712499 (PMC) (EuropePMC)
Related Publication(s)
Note

Predicting cell shapes in melanomas.
Tyrrell et al., 2014, Pigment Cell Melanoma Res. 27(1): 5--6 [FBrf0225943]

Finding the shape-shifter genes.
Olson, 2013, Nat. Cell Biol. 15(7): 723--725 [FBrf0221985]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Cell Biol.
    Title
    Nature Cell Biology
    Publication Year
    1999-
    ISBN/ISSN
    1465-7392 1476-4679
    Data From Reference