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Citation
Ilik, I.A., Quinn, J.J., Georgiev, P., Tavares-Cadete, F., Maticzka, D., Toscano, S., Wan, Y., Spitale, R.C., Luscombe, N., Backofen, R., Chang, H.Y., Akhtar, A. (2013). Tandem Stem-Loops in roX RNAs Act Together to Mediate X Chromosome Dosage Compensation in Drosophila.  Mol. Cell 51(2): 156--173.
FlyBase ID
FBrf0222154
Publication Type
Research paper
Abstract
Dosage compensation in Drosophila is an epigenetic phenomenon utilizing proteins and long noncoding RNAs (lncRNAs) for transcriptional upregulation of the male X chromosome. Here, by using UV crosslinking followed by deep sequencing, we show that two enzymes in the Male-Specific Lethal complex, MLE RNA helicase and MSL2 ubiquitin ligase, bind evolutionarily conserved domains containing tandem stem-loops in roX1 and roX2 RNAs in vivo. These domains constitute the minimal RNA unit present in multiple copies in diverse arrangements for nucleation of the MSL complex. MLE binds to these domains with distinct ATP-independent and ATP-dependent behavior. Importantly, we show that different roX RNA domains have overlapping function, since only combinatorial mutations in the tandem stem-loops result in severe loss of dosage compensation and consequently male-specific lethality. We propose that repetitive structural motifs in lncRNAs could provide plasticity during multiprotein complex assemblies to ensure efficient targeting in cis or in trans along chromosomes.
Graphical Abstract
Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC3804161 (PMC) (EuropePMC)
Related Publication(s)
Note
Noncoding roX RNA Remodeling Triggers Fly Dosage Compensation Complex Assembly.
Wutz, 2013, Mol. Cell 51(2): 131--132 [FBrf0222191]
Non-coding RNA: Structure and function for lncRNAs.
Flintoft, 2013, Nat. Rev. Genet. 14(9): 598 [FBrf0223562]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Cell
    Title
    Molecular Cell
    Publication Year
    1997-
    ISBN/ISSN
    1097-2765 1097-4164
    Data From Reference
    Aberrations (1)
    Alleles (18)
    Genes (10)
    Physical Interactions (12)
    Cell Lines (2)
    Natural transposons (1)
    Insertions (14)
    Experimental Tools (1)
    Transgenic Constructs (16)