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Mockett, R.J., Nobles, A.C. (2013). Lack of Robustness of Life Extension Associated With Several Single-Gene P Element Mutations in Drosophila melanogaster.  J. Gerontol. A Biol. Sci. Med. Sci. 68(10): 1157--1169.
FlyBase ID
FBrf0222732
Publication Type
Research paper
Abstract

The hypothesis tested in this study was that single-gene mutations found previously to extend the life span of Drosophila melanogaster could do so consistently in both long-lived y w and standard w (1118) genetic backgrounds. GAL4 drivers were used to express upstream activation sequence (UAS)-responder transgenes globally or in the nervous system. Transgenes associated with oxidative damage prevention (UAS-hSOD1 and UAS-GCLc) or removal (EP-UAS-Atg8a and UAS-dTOR (FRB) ) failed to increase mean life spans in any expression pattern in either genetic background. Flies containing a UAS-EGFP-bMSRA (C) transgene associated with protein repair were found not to exhibit life extension or detectable enhanced green fluorescent protein (EGFP) activity. The presence of UAS-responder transgenes was confirmed by PCR amplification and sequencing at the 5' and 3' end of each insertion. These results cast doubt on the robustness of life extension in flies carrying single-gene mutations and suggest that the effects of all such mutations should be tested independently in multiple genetic backgrounds and laboratory environments.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Gerontol. A Biol. Sci. Med. Sci.
    Title
    The journals of gerontology. Series A, Biological sciences and medical sciences
    Publication Year
    1994-
    ISBN/ISSN
    1079-5006 1758-535X
    Data From Reference
    Genes (4)