Open Close
Reference
Citation
Dokudovskaya, S., Waharte, F., Schlessinger, A., Pieper, U., Devos, D.P., Cristea, I.M., Williams, R., Salamero, J., Chait, B.T., Sali, A., Field, M.C., Rout, M.P., Dargemont, C. (2011). A Conserved Coatomer-related Complex Containing Sec13 and Seh1 Dynamically Associates With the Vacuole in Saccharomyces cerevisiae.  Mol. Cell. Proteomics 10(6): M110.006478.
FlyBase ID
FBrf0223060
Publication Type
Research paper
Abstract

The presence of multiple membrane-bound intracellular compartments is a major feature of eukaryotic cells. Many of the proteins required for formation and maintenance of these compartments share an evolutionary history. Here, we identify the SEA (Seh1-associated) protein complex in yeast that contains the nucleoporin Seh1 and Sec13, the latter subunit of both the nuclear pore complex and the COPII coating complex. The SEA complex also contains Npr2 and Npr3 proteins (upstream regulators of TORC1 kinase) and four previously uncharacterized proteins (Sea1-Sea4). Combined computational and biochemical approaches indicate that the SEA complex proteins possess structural characteristics similar to the membrane coating complexes COPI, COPII, the nuclear pore complex, and, in particular, the related Vps class C vesicle tethering complexes HOPS and CORVET. The SEA complex dynamically associates with the vacuole in vivo. Genetic assays indicate a role for the SEA complex in intracellular trafficking, amino acid biogenesis, and response to nitrogen starvation. These data demonstrate that the SEA complex is an additional member of a family of membrane coating and vesicle tethering assemblies, extending the repertoire of protocoatomer-related complexes.

PubMed ID
PubMed Central ID
PMC3108837 (PMC) (EuropePMC)
Related Publication(s)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Cell. Proteomics
    Title
    Molecular and Cellular Proteomics
    Publication Year
    2002-
    ISBN/ISSN
    1535-9476
    Data From Reference
    Gene Groups (3)
    Genes (8)