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Nemčovičová, I., Sesták, S., Rendić, D., Plšková, M., Mucha, J., Wilson, I.B. (2013). Characterisation of class I and II α-mannosidases from Drosophila melanogaster.  Glycoconjugate J. 30(9): 899--909.
FlyBase ID
FBrf0223118
Publication Type
Research paper
Abstract
Homology searches indicated that up to five class I α-mannosidases (glycohydrolase family 47) and eight class II α-mannosidases (glycohydrolase family 38) are encoded by the fruitfly (Drosophila melanogaster) genome. Selected example mannosidases were expressed in secreted form using the yeast Pichia pastoris. A number of characteristics of these enzymes were determined with p-nitrophenyl-α-mannoside as substrate; particularly striking were the low optima (pH 5) of three class II mannosidases most closely related to known lysosomal mannosidases and the distinct Co(II)-requirement of a mannosidase previously named ManIIb. Some of the recombinant mannosidases were demonstrably active towards oligomannosidic glycans, specifically, the Co(II)-requiring ManIIb, two 'acidic' mannosidases and the class I mas-1 mannosidase. Other than previous characterisations of the well-known Golgi mannosidase II, this is the first study summarising various properties of recombinant mannosidases from the fruitfly.
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Glycoconjugate J.
    Title
    Glycoconjugate Journal
    Publication Year
    1984-
    ISBN/ISSN
    0282-0080
    Data From Reference