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Citation
Kwon, Y., Vinayagam, A., Sun, X., Dephoure, N., Gygi, S.P., Hong, P., Perrimon, N. (2013). The Hippo signaling pathway interactome.  Science 342(6159): 737--740.
FlyBase ID
FBrf0223213
Publication Type
Research paper
Abstract
The Hippo pathway controls metazoan organ growth by regulating cell proliferation and apoptosis. Many components have been identified, but our knowledge of the composition and structure of this pathway is still incomplete. Using existing pathway components as baits, we generated by mass spectrometry a high-confidence Drosophila Hippo protein-protein interaction network (Hippo-PPIN) consisting of 153 proteins and 204 interactions. Depletion of 67% of the proteins by RNA interference regulated the transcriptional coactivator Yorkie (Yki) either positively or negatively. We selected for further characterization a new member of the alpha-arrestin family, Leash, and show that it promotes degradation of Yki through the lysosomal pathway. Given the importance of the Hippo pathway in tumor development, the Hippo-PPIN will contribute to our understanding of this network in both normal growth and cancer.
PubMed ID
PubMed Central ID
PMC3951131 (PMC) (EuropePMC)
Related Publication(s)
Note
Hippo unleashed! Proteome-scale analysis reveals new views of Hippo pathway biology.
Weiss, 2013, Sci. Signal. 6(302): pe36 [FBrf0225789]
Discovering the Hippo pathway protein-protein interactome.
Moya and Halder, 2014, Cell Res. 24(2): 137--138 [FBrf0226302]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Science
    Title
    Science
    Publication Year
    1895-
    ISBN/ISSN
    0036-8075
    Data From Reference
    Alleles (8)
    Genes (173)
    Physical Interactions (24)
    Datasets (5)
    Cell Lines (1)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (1)
    Transgenic Constructs (7)