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Citation
Link, N., Kurtz, P., O'Neal, M., Garcia-Hughes, G., Abrams, J.M. (2013). A p53 enhancer region regulates target genes through chromatin conformations in cis and in trans.  Genes Dev. 27(22): 2433--2438.
FlyBase ID
FBrf0223347
Publication Type
Research paper
Abstract

We examined how a p53 enhancer transmits regulatory information in vivo. Using genetic ablation together with digital chromosome conformation capture and fluorescent in situ hybridization, we found that a Drosophila p53 enhancer region (referred to as the p53 response element [p53RE]) physically contacts targets in cis and across the centromere to control stress-responsive transcription at these sites. Furthermore, when placed at ectopic genomic positions, fragments spanning this element re-established chromatin contacts and partially restored target gene regulation to mutants lacking the native p53RE. Therefore, a defined p53 enhancer region is sufficient for long-range chromatin interactions that enable multigenic regulation.

PubMed ID
PubMed Central ID
PMC3841732 (PMC) (EuropePMC)
Related Publication(s)
Note

DNA loops specify p53 network responses.
Link and Abrams, 2014, Cell Cycle 13(11): 1659 [FBrf0227216]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Dev.
    Title
    Genes & Development
    Publication Year
    1987-
    ISBN/ISSN
    0890-9369
    Data From Reference
    Aberrations (1)
    Alleles (2)
    Genes (6)
    Natural transposons (1)
    Transgenic Constructs (2)