Shinya Yamamoto*, Manish Jaiswal*, Hector Sandoval, Vafa Bayat, Ke Zhang, Bo Xiong, Wu-Lin Charng, Nele A. Haelterman, Lichun Jiang, Yumei Li, Gabriela David, Tongchao Li, Kuchuan Chen, Upasana Gala, Adeel Jawaid, Stephen Gibbs, Danqing Bei, Hsiang-Chih Lu, Theodore Busby, Alexander Lin-Moore, Karen L. Schulze, Rui Chen and Hugo J Bellen. 
*Equal contribution
This collection was generated by the Bellen lab as part of a forward genetic screen on the X chromosome which utilized low concentrations of ethyl methane sulfonate to minimize lethal hits per chromosome. More detailed methods are presented here: FBrf0220110, FBrf0221023, FBrf0220111, FBrf0222390 (see also Shinya Yamamoto, Manish Jaiswal et al., Large scale isolation of genes affecting neuronal development, function, or maintenance in Drosophila. In preparation). Briefly, y1 w* P{ry+neoFRT}19A chromosomes were mutagenized and balanced stocks were established. Selection was based on phenotypic characterization of homozygous mutant tissues in two types of clones – electroretinograms of ey-FLP eye clones and morphological observation of wings and thoraces of Ubx-FLP clones. Complementation tests, duplication mapping and Pacman mapping were performed, and mutations in selected cases were determined by PCR and Sanger sequencing and/or whole genome sequencing.