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Inagaki, H., Anderson, D. (2013.11.13). ReaChR stocks. 
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FBrf0223546
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Personal communication to FlyBase
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The following information accompanied stocks donated to the Bloomington Stock Center by Hidehiko Inagaki and David Anderson, California Institute of Technology.
P{UAS-ReaChR} expresses a citrine-tagged channelrhodopsin variant (ReaChR) under UAS control. ReaChR is activated by light farther toward red than channelrhodopsin variants activated by blue light.  Red light penetrates the cuticle better than blue light.  P{UAS-ReaChR} is inserted in a P element-based attP site.
PBac{UAS-ReaChR} is the same construct inserted in a PBac-based attP target site.
P{lexAop-ReaChR} expresses the same protein under lexAop control.
PBac{lexAop-ReaChR} is the same construct inserted in a PBac-based attP target site.
P{UAS(FRT.mCherry)ReaChR} is similar to P{UAS-ReaChR} except it carries an FRT-mCherry-FRT cassette interposed between UAS and ReaChR sequences.  The cassette contains a stop codon positioned after the mCherry sequence. P{UAS(FRT.mCherry)ReaChR}is inserted in a P element-based attP site.
PBac{UAS(FRT.mCherry)ReaChR} is the same construct inserted in a PBac-based attP target site.
P{lexAop(FRT.mCherry)ReaChR} is similar to P{UAS(FRT.mCherry)ReaChR} with the substitution of lexAop for UAS.  P{lexAop(FRT.mCherry)ReaChR} is inserted in a P element-based attP site.
PBac{lexAop(FRT.mCherry)ReaChR} is the same construct inserted in a PBac-based attP target site.
P{UAS-ReaChR}attP40 and P{lexAop-ReaChR}attP40 are insertions into P{CaryP}attP40 at 25C6,  2L:5108448  (R5).
PBac{UAS-ReaChR}VK00005, PBac{lexAop-ReaChR}VK00005, PBac{UAS(FRT.mCherry)ReaChR}VK00005 and PBac{lexAop(FRT.mCherry)ReaChR}VK00005 are insertions into PBac{y+-attP-9A}VK00005 at 75A10,  3L:17945208  (R5).
P{UAS-ReaChR}su(Hw)attP5, P{lexAop-ReaChR}su(Hw)attP5, P{UAS(FRT.mCherry)ReaChR}su(Hw)attP5 and P{lexAop(FRT.mCherry)ReaChR}su(Hw)attP5 are insertions into P{CaryIP}su(Hw)attP5 at 50F1,  2R:10194873..10195195  (R5).
--
Kevin Cook, Ph.D.
Bloomington Drosophila Stock Center
http://flystocks.bio.indiana.edu
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Related Publication(s)
Research paper
Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship.
Inagaki et al., 2014, Nat. Methods 11(3): 325--332 [FBrf0224262]
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