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Citation
Cassidy, J.J., Jha, A.R., Posadas, D.M., Giri, R., Venken, K.J., Ji, J., Jiang, H., Bellen, H.J., White, K.P., Carthew, R.W. (2013). miR-9a Minimizes the Phenotypic Impact of Genomic Diversity by Buffering a Transcription Factor.  Cell 155(7): 1556--1567.
FlyBase ID
FBrf0223740
Publication Type
Research paper
Abstract

Gene expression has to withstand stochastic, environmental, and genomic perturbations. For example, in the latter case, 0.5%-1% of the human genome is typically variable between any two unrelated individuals. Such diversity might create problematic variability in the activity of gene regulatory networks and, ultimately, in cell behaviors. Using multigenerational selection experiments, we find that for the Drosophila proneural network, the effect of genomic diversity is dampened by miR-9a-mediated regulation of senseless expression. Reducing miR-9a regulation of the Senseless transcription factor frees the genomic landscape to exert greater phenotypic influence. Whole-genome sequencing identified genomic loci that potentially exert such effects. A larger set of sequence variants, including variants within proneural network genes, exhibits these characteristics when miR-9a concentration is reduced. These findings reveal that microRNA-target interactions may be a key mechanism by which the impact of genomic diversity on cell behavior is dampened.

Graphical Abstract
Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC3891883 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Aberrations (1)
    Alleles (10)
    Genes (5)
    Natural transposons (1)
    Insertions (1)
    Transgenic Constructs (4)