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Citation
Antonucci, L., D'Amico, D., Di Magno, L., Coni, S., Di Marcotullio, L., Cardinali, B., Gulino, A., Ciapponi, L., Canettieri, G. (2014). CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMyc.  Cell Cycle 13(3): 434--439.
FlyBase ID
FBrf0224112
Publication Type
Research paper
Abstract

CCHC-type zinc finger nucleic acid binding protein (CNBP) is a small conserved protein, which plays a key role in development and disease. Studies in animal models have shown that the absence of CNBP results in severe developmental defects that have been mostly attributed to its ability to regulate c-myc mRNA expression. Functionally, CNBP binds single-stranded nucleic acids and acts as a molecular chaperone, thus regulating both transcription and translation.   In this work we report that in Drosophila melanogaster, CNBP is an essential gene, whose absence causes early embryonic lethality. In contrast to what observed in other species, ablation of CNBP does not affect dMyc mRNA expression, whereas the protein levels are markedly reduced. We demonstrate for the first time that dCNBP regulates dMyc translation through an IRES-dependent mechanism, and that knockdown of dCNBP in the wing territory causes a general reduction of wing size, in keeping with the reported role of dMyc in this region. Consistently, reintroduction of dMyc in CNBP-deficient wing imaginal discs rescues the wing size, further supporting a key role of the CNBP-Myc axis in this context. Collectively, these data show a previously uncharacterized mechanism, whereby, by regulating dMyc IRES-dependent translation, CNBP controls Drosophila wing development. These results may have relevant implications in other species and in pathophysiological conditions.

PubMed ID
PubMed Central ID
PMC3956539 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Cycle
    Title
    Cell Cycle
    Publication Year
    2002
    ISBN/ISSN
    1538-4101 1551-4005
    Data From Reference
    Alleles (6)
    Genes (3)
    Insertions (3)
    Transgenic Constructs (5)