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Citation
Heinrichsen, E.T., Zhang, H., Robinson, J.E., Ngo, J., Diop, S., Bodmer, R., Joiner, W.J., Metallo, C.M., Haddad, G.G. (2014). Metabolic and transcriptional response to a high-fat diet in Drosophila melanogaster.  Mol. Metab. 3(1): 42--54.
FlyBase ID
FBrf0224147
Publication Type
Research paper
Abstract

Obesity has dramatically increased in prevalence, making it essential to understand its accompanying metabolic changes. Modeling diet-induced obesity in Drosophila melanogaster (fruit flies), we elucidated transcriptional and metabolic changes in w (1118) flies on a high-fat diet (HFD). Mass spectrometry-based metabolomics revealed altered fatty acid, amino acid, and carbohydrate metabolism with HFD. Microarray analysis uncovered transcriptional changes in nitrogen metabolism, including CG9510, homolog of human argininosuccinate lyase (ASL). CG9510 knockdown in flies phenocopied traits observed with HFD, namely increased triglyceride levels and decreased cold tolerance. Restoration of CG9510 expression ameliorated observed negative consequences of HFD. Metabolomic analysis of CG9510 knockdown flies confirmed functional similarity to ASL, regulating the balance of carbon and nitrogen metabolism. In summary, we found that HFD suppresses CG9510 expression, a gene required for proper triglyceride storage and stress tolerance. These results draw an important link between regulation of amino acid metabolism and the response to diet-induced obesity.

PubMed ID
PubMed Central ID
PMC3929909 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Metab.
    Title
    Molecular metabolism
    ISBN/ISSN
    2212-8778
    Data From Reference
    Genes (13)
    Human Disease Models (1)