Questions from FlyBase curator: In a few cases, the expected residue was not found at the reported location in the reference sequence for the X lethal mutation. Please clarify. 1. Query: shiA - Amino acid replacement: K28@. residue 28 is Q in all isoforms Answer: This should be K10@ 2. Query: Nmd3A - Amino acid replacement: V4516E. Nmd3 is only 521 aa long, must be typo. There is a V at 416 Answer: Indeed, it is supposed to be 416. 3. Query: hopC - Amino acid replacement: Q115@. nearest Q is at 119, residue 115 is R (TGA) Answer: Would it be possible for you to check whether in release 5.12 of the reference genome, the 115th amino acid of hopscotch is a Q and whether this represents Q119 in the current release? The raw sequence file for this mutant has been corrupted and I cannot check the sequence that surrounds the mutation. Response: The hop protein sequence is the same in release R5.12 as in R5.56. 4. Query: ewgB - Amino acid replacement: Q80@. no Q at 80, residue 80 is G (GGA) Answer: It is Q166@ 5. Query: elavB - Amino acid replacement: Q364@. no Q at 364 in any isoforms. Answer: This should be Q122@ 6. Query: DAAMA - Amino acid replacement: N360V. no N at residue 360 in any isoforms Answer: This should be D360V. 7. Query: COQ7B - Amino acid replacement: Y90@. residue 90 is a W, no Y nearby Answer: This is supposed to be W90@ 8. Query: Aats-hisA - Amino acid replacement: D160V. no D at residue 160 in any of four different isoforms Answer: This becomes D119V (I think with the new version of the genome? We annotated our mutations on the 3rd version of the reference sequence.