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Victorsen, A., White, K. (2014.4.15). Victorsen insertions. 
FlyBase ID
FBrf0224688
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Personal communication to FlyBase
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Text of Personal Communication
The following information and information in a linked spreadsheet accompanied stocks donated to the Bloomington Stock Center by Alec Victorsen and Kevin White, University of Chicago.
BAC clones were recombineered to introduce sequences encoding fluorescence or epitope tags at specific positions within genes to tag specific protein isoforms. The clones were integrated at specific attP sites by phiC31 integrase-mediated transformation. Though the constructs are named for the tagged gene, other genes are often present in the constructs.
In the construct names, 'GFP.FPTB' represents combinations of epitope tags and peptide sequences:
GFP.FPTB is a C-terminal superfolderGFP-FLAG-PreScission-TEV-BLRP tag combination.
Information about the protein isoforms tagged by each construct is included in the downloadable spreadsheet.
The following insertions are described in the spreadsheet.
PBac{lbe-GFP.FPTB}VK00037
PBac{polybromo-GFP.FPTB}VK00037
PBac{CG33213-GFP.FPTB}VK00037
PBac{emc-GFP.FPTB}VK00037
PBac{Kr-h2-GFP.FPTB}VK00033
PBac{HmgD-GFP.FPTB}VK00033
PBac{achi-GFP.FPTB}VK00033
PBac{Crg-1-GFP.FPTB}VK00033
PBac{sage-GFP.FPTB}VK00037
PBac{Elba2-GFP.FPTB}VK00033
PBac{CG12744-GFP.FPTB}VK00033
PBac{CG9305-GFP.FPTB}VK00033
PBac{CG10147-GFP.FPTB}VK00037
PBac{CG33557-GFP.FPTB}VK00033
PBac{da-GFP.FPTB}VK00033
PBac{tx-GFP.FPTB}VK00037
PBac{cyc-GFP.FPTB}VK00037
PBac{E(spl)m7-HLH-GFP.FPTB}VK00037
PBac{CG3919-GFP.FPTB}VK00037
PBac{HLH54F-GFP.FPTB}VK00033
PBac{Sox14-GFP.FPTB}VK00033
PBac{CG4854-GFP.FPTB}VK00037
PBac{CG4282-GFP.FPTB}VK00033
PBac{CG7045-GFP.FPTB}VK00037 (FlyBase curator comment: symbol given in column Q of the associated spreadsheet incorrectly implies that this insertion was made into the VK00033 docking site, but the target site was the VK00037 docking site, as correctly indicated for this line in columns D and E of the spreadsheet).
DOI
Associated Information
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Associated Files
File date: 2014.4.15 ; File size: 12524 ; File format: xlsx ; File name: Victorsen.2014.4.15.xlsx
Other Information
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    Language of Publication
    English
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    Data From Reference
    Alleles (24)
    Genes (24)
    Natural transposons (1)
    Insertions (25)
    Experimental Tools (5)
    Transgenic Constructs (24)