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Citation
Marelja, Z., Dambowsky, M., Bolis, M., Georgiou, M.L., Garattini, E., Missirlis, F., Leimk├╝hler, S. (2014). The four aldehyde oxidases of Drosophila melanogaster have different gene expression patterns and enzyme substrate specificities.  J. Exp. Biol. 217(12): 2201--2211.
FlyBase ID
FBrf0225357
Publication Type
Research paper
Abstract

In the genome of Drosophila melanogaster, four genes coding for aldehyde oxidases (AOX1-4) were identified on chromosome 3. Phylogenetic analysis showed that the AOX gene cluster evolved via independent duplication events in the vertebrate and invertebrate lineages. The functional role and the substrate specificity of the distinct Drosophila AOX enzymes is unknown. Two loss-of-function mutant alleles in this gene region, low pyridoxal oxidase (Po(lpo)) and aldehyde oxidase-1 (Aldox-1(n1)) are associated with a phenotype characterized by undetectable AOX enzymatic activity. However, the genes involved and the corresponding mutations have not yet been identified. In this study we characterized the activities, substrate specificities and expression profiles of the four AOX enzymes in D. melanogaster. We show that the Po(lpo)-associated phenotype is the consequence of a structural alteration of the AOX1 gene. We identified an 11-bp deletion in the Po(lpo) allele, resulting in a frame-shift event, which removes the molybdenum cofactor domain of the encoded enzyme. Furthermore, we show that AOX2 activity is detectable only during metamorphosis and characterize a Minos-AOX2 insertion in this developmental gene that disrupts its activity. We demonstrate that the Aldox-1(n1) phenotype maps to the AOX3 gene and AOX4 activity is not detectable in our assays.

PubMed ID
PubMed Central ID
PMC4055135 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Location data for AOX1[lpo] deletion.
Missirlis and Marelja, 2014.1.10, Location data for AOX1[lpo] deletion. [FBrf0228281]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Exp. Biol.
    Title
    Journal of Experimental Biology
    Publication Year
    1930-
    ISBN/ISSN
    0022-0949
    Data From Reference
    Aberrations (3)
    Alleles (7)
    Gene Groups (1)
    Genes (7)
    Transgenic Constructs (3)