Open Close
Reference
Citation
Huang, W., Massouras, A., Inoue, Y., Peiffer, J., RĂ mia, M., Tarone, A.M., Turlapati, L., Zichner, T., Zhu, D., Lyman, R.F., Magwire, M.M., Blankenburg, K., Carbone, M.A., Chang, K., Ellis, L.L., Fernandez, S., Han, Y., Highnam, G., Hjelmen, C.E., Jack, J.R., Javaid, M., Jayaseelan, J., Kalra, D., Lee, S., Lewis, L., Munidasa, M., Ongeri, F., Patel, S., Perales, L., Perez, A., Pu, L., Rollmann, S.M., Ruth, R., Saada, N., Warner, C., Williams, A., Wu, Y.Q., Yamamoto, A., Zhang, Y., Zhu, Y., Anholt, R.R., Korbel, J.O., Mittelman, D., Muzny, D.M., Gibbs, R.A., Barbadilla, A., Johnston, J.S., Stone, E.A., Richards, S., Deplancke, B., Mackay, T.F. (2014). Natural variation in genome architecture among 205 Drosophila melanogaster Genetic Reference Panel lines.  Genome Res. 24(7): 1193--1208.
FlyBase ID
FBrf0225536
Publication Type
Research paper
Abstract

The Drosophila melanogaster Genetic Reference Panel (DGRP) is a community resource of 205 sequenced inbred lines, derived to improve our understanding of the effects of naturally occurring genetic variation on molecular and organismal phenotypes. We used an integrated genotyping strategy to identify 4,853,802 single nucleotide polymorphisms (SNPs) and 1,296,080 non-SNP variants. Our molecular population genomic analyses show higher deletion than insertion mutation rates and stronger purifying selection on deletions. Weaker selection on insertions than deletions is consistent with our observed distribution of genome size determined by flow cytometry, which is skewed toward larger genomes. Insertion/deletion and single nucleotide polymorphisms are positively correlated with each other and with local recombination, suggesting that their nonrandom distributions are due to hitchhiking and background selection. Our cytogenetic analysis identified 16 polymorphic inversions in the DGRP. Common inverted and standard karyotypes are genetically divergent and account for most of the variation in relatedness among the DGRP lines. Intriguingly, variation in genome size and many quantitative traits are significantly associated with inversions. Approximately 50% of the DGRP lines are infected with Wolbachia, and four lines have germline insertions of Wolbachia sequences, but effects of Wolbachia infection on quantitative traits are rarely significant. The DGRP complements ongoing efforts to functionally annotate the Drosophila genome. Indeed, 15% of all D. melanogaster genes segregate for potentially damaged proteins in the DGRP, and genome-wide analyses of quantitative traits identify novel candidate genes. The DGRP lines, sequence data, genotypes, quality scores, phenotypes, and analysis and visualization tools are publicly available.

PubMed ID
PubMed Central ID
PMC4079974 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genome Res.
    Title
    Genome Research
    Publication Year
    1995-
    ISBN/ISSN
    1088-9051
    Data From Reference
    Aberrations (16)
    Genes (4)
    Datasets (1)
    Strains (205)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.