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Bischof, J., FlyORF project members, (2014.6.20). Description of new FlyORF insertion lines made using the pGW-HA.attB FlyORF vector. 
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FBrf0225611
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Personal communication to FlyBase
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The transgenic lines were created by use of the phiC31 integrase method, targeting the transgenes to the landing site ZH-attP-86Fb on chromosome 3R. This landing site was created with the construct pM{3xP3-RFPattP} that is marked by 3xP3-RFP. This attP landing site is located within cytological region 86F (genomic position: 7634080/7634081) in the 5' intron of the two longer isoforms of the gene chloride channel-a (ClC-a; CG31116). This site displays a relatively strong Gal4-dependent transgene expression. We have detected some basal expression at this site during early embryonic development, thus not all inserted transgenes resulted in homozygous stocks.
The UAS-ORF-3xHA transgenes were inserted with the pGW-HA.attB vector, i.e. all the ORFs are C-terminal tagged with the 3xHA epitope. Note, all UAS-ORF-3xHA transgenes created with the pGW-HA.attB vector allow exchange of either the promoter or the epitope tag by crossing the UAS-ORF-3XHA line with either epitope tag or promoter 'swapping' lines (FLP/FRT-mediated in vivo exchange).
Detailed information on all these issues can be found on the FlyORF website and in the referenced literature. 
DOI
Related Publication(s)
Research paper
A versatile platform for creating a comprehensive UAS-ORFeome library in Drosophila.
Bischof et al., 2013, Development 140(11): 2434--2442 [FBrf0221597]
Generation of a transgenic ORFeome library in Drosophila.
Bischof et al., 2014, Nat. Protoc. 9(7): 1607--1620 [FBrf0225367]
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Associated Files
File date: 2014.7.10 ; File size: 57552 ; File format: xlsx ; File name: new_FlyORF_insertion_lines.xlsx
File date: 2014.9.24 ; File size: 48246 ; File format: xlsx ; File name: FlyORF_Lines_Sep2014.xlsx
File date: 2015.4.8 ; File size: 76804 ; File format: xlsx ; File name: FlyORF_Lines_Apr2015.xlsx
File date: 2016.2.5 ; File size: 47146 ; File format: xlsx ; File name: FlyORF_Lines_Feb2016.xlsx
File date: 2019.5.21 ; File size: 25401 ; File format: xlsx ; File name: FlyORF_Lines_2019.5.21.xlsx
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    English
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