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Kim, S.N., Jeibmann, A., Halama, K., Witte, H.T., Wälte, M., Matzat, T., Schillers, H., Faber, C., Senner, V., Paulus, W., Klämbt, C. (2014). ECM stiffness regulates glial migration in Drosophila and mammalian glioma models.  Development 141(16): 3233--3242.
FlyBase ID
FBrf0225820
Publication Type
Research paper
Abstract

Cell migration is an important feature of glial cells. Here, we used the Drosophila eye disc to decipher the molecular network controlling glial migration. We stimulated glial motility by pan-glial PDGF receptor (PVR) activation and identified several genes acting downstream of PVR. Drosophila lox is a non-essential gene encoding a secreted protein that stiffens the extracellular matrix (ECM). Glial-specific knockdown of Integrin results in ECM softening. Moreover, we show that lox expression is regulated by Integrin signaling and vice versa, suggesting that a positive-feedback loop ensures a rigid ECM in the vicinity of migrating cells. The general implication of this model was tested in a mammalian glioma model, where a Lox-specific inhibitor unraveled a clear impact of ECM rigidity in glioma cell migration.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Development
    Title
    Development
    Publication Year
    1987-
    ISBN/ISSN
    0950-1991
    Data From Reference
    Genes (31)
    Cell Lines (1)