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Citation
Komori, H., Xiao, Q., Janssens, D.H., Dou, Y., Lee, C.Y. (2014). Trithorax maintains the functional heterogeneity of neural stem cells through the transcription factor Buttonhead.  eLife 3(): e03502.
FlyBase ID
FBrf0226697
Publication Type
Research paper
Abstract

The mechanisms that maintain the functional heterogeneity of stem cells, which generates diverse differentiated cell types required for organogenesis, are not understood. In this study, we report that Trithorax (Trx) actively maintains the heterogeneity of neural stem cells (neuroblasts) in the developing Drosophila larval brain. trx mutant type II neuroblasts gradually adopt a type I neuroblast functional identity, losing the competence to generate intermediate neural progenitors (INPs) and directly generating differentiated cells. Trx regulates a type II neuroblast functional identity in part by maintaining chromatin in the buttonhead (btd) locus in an active state through the histone methyltransferase activity of the SET1/MLL complex. Consistently, btd is necessary and sufficient for eliciting a type II neuroblast functional identity. Furthermore, over-expression of btd restores the competence to generate INPs in trx mutant type II neuroblasts. Thus, Trx instructs a type II neuroblast functional identity by epigenetically promoting Btd expression, thereby maintaining neuroblast functional heterogeneity.

PubMed ID
PubMed Central ID
PMC4221733 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Location data for Rbbp5[null] excision deletion.
Komori and Lee, 2016.8.10, Location data for Rbbp5[null] excision deletion. [FBrf0233101]

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Aberrations (1)
    Alleles (20)
    Genes (16)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (3)
    Transgenic Constructs (6)
    Transcripts (1)